Biophysical Determinants of Toluene Diisocyanate Antigenicity Associated with Exposure and Asthma

Details

• Personal Author:
  Kim CW ; Kim HM ; Kim HR ; Nahm DH ; Park HS ; Redlich CA ; Suh CH ; Wisnewski AV ; Ye YM
• Description:
  BACKGROUND: Toluene diisocyanate (TDI), a widely used aromatic diisocyanate with the potential to cause asthma, reacts with albumin in the airway fluid, which acts as a carrier protein for chemical presentation to the immune system. Structural elucidation of TDI-albumin conjugates is crucial to understanding the human immune response to TDI exposure. OBJECTIVE: Investigate the dependence of TDI's antigenicity on the biophysics of exposure and its association with TDI asthma. METHODS: Toluene diisocyanate-albumin conjugates were generated by exposing albumin to TDI in liquid or vapor phase (liquid or vapor TDI-albumin, respectively). Conjugates were characterized by native gel electrophoresis and matrix-assisted laser desorption/ionization-mass spectrometry, and used as antigens in ELISA assays for serum specific-IgE and IgG. RESULTS: The physical phase of TDI (vapor vs liquid) affects the formation of TDI-albumin conjugates, with measurable differences in the amount of TDI per albumin molecule, migration in native gels, matrix-assisted laser desorption/ionization-mass spectrometry mass/charge spectra, and antigenicity. Vapor TDI-albumin conjugates were recognized by IgE from 44% of subjects with TDI asthma, whereas liquid TDI-albumin conjugates are recognized by IgE from only 17% of these patients. A significant (P < .05) association between TDI exposure and vapor TDI-albumin specific serum IgG was also observed. CONCLUSION: Biophysics of TDI exposure substantially affects formation of TDI-albumin conjugates recognized by the immune system in association with exposure and asthma. CLINICAL IMPLICATIONS: The data suggest that serology may help identify TDI asthmatics and exposed workers if the appropriate form of TDI is used as the antigenic basis for analysis. [Description provided by NIOSH]
• Subjects:
  Hypersensitivity Immune System Occupational Health Respiratory Tract Diseases Safety
• Keywords:
  Airway-obstruction Allergens Exposure-levels Immunologic-disorders Immunotoxins Pathogenesis Pulmonary-system-disorders Respiratory-system-disorders
• ISSN:
  0091-6749
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Connecticut ; OSHA Region 1
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  118
• Issue:
  4
• NIOSHTIC Number:
  nn:20031331
• Citation:
  J Allergy Clin Immunol 2006 Oct; 118(4):885-891
• Contact Point Address:
  Department of Allergy and Rheumatology, Ajou University School of Medicine, Suwon, Korea 442-721
• CAS Registry Number:
  Toluene 2,4-diisocyanate (CAS RN 584-84-9)
• Federal Fiscal Year:
  2007
• NORA Priority Area:
  Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
• Performing Organization:
  Yale University, New Haven, Connecticut
• Peer Reviewed:
  True
• Start Date:
  19970901
• Source Full Name:
  Journal of Allergy and Clinical Immunology
• End Date:
  20020831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0c757e4672849a65a4be7a4d00703be1fa8aef4709eeb5a46131d5955567904a493b738c6e9862e8d77adca8ef7ffd593e1bad0158b2bee78a184e818d35db67
• Download URL:
  https://stacks.cdc.gov/view/cdc/191602/cdc_191602_DS1.pdf
• File Type:
  [PDF - 331.14 KB ]