Exhaled Nitric Oxide Measurement in Workers in a Microwave Popcorn Production Plant

Details

• Personal Author:
  Carroll N ; James A ; Janssen Y ; Kaminsky DA ; Mitchell J ; Soultanakis R
• Description:
  BACKGROUND: Recent evidence has shown that nitric oxide (NO) levels are increased in asthmatic airways. Although the role of NO in asthma is unknown, reactive metabolites of NO may lead to nitrotyrosine formation and promote airway dysfunction. OBJECTIVE: The aim of this study was to determine whether nitrotyrosine, as a marker of nitrating species, could be found in the airways and lung parenchyma of subjects with asthma who died of status asthmaticus or other nonrespiratory causes. METHODS: Lung tissue specimens were obtained from 5 patients who died of status asthmaticus, 2 asthmatic patients who died of nonrespiratory causes, and 6 nonasthmatic control subjects who died of nonrespiratory causes. Lung sections were stained for immunofluorescence with use of an antinitrotyrosine antibody, followed by a indiocarbocyanine (Cy5, Jackson Immunochemicals, Westgrove, Pa)-conjugated secondary antibody. RESULTS: Nonasthmatic lungs showed little or no nitrotyrosine staining, whereas asthmatic lungs demonstrated significantly more staining of nitrotyrosine residues distributed in both the airways and lung parenchyma. CONCLUSION: This study demonstrates the presence of nitrotyrosine, and hence evidence of formation of nitrating species, in the airways and lung parenchyma of patients with asthma who died of status asthmaticus or other nonrespiratory causes. This finding supports the concept that widespread airway and parenchymal inflammation occurs in asthma, and, more specifically, that NO and its reactive metabolites may play a pathophysiologic role in asthma. [Description provided by NIOSH]
• Subjects:
  Animals Hypersensitivity Occupational Health Respiratory Tract Diseases Safety
• Keywords:
  Airway-obstruction Allergens Animal-studies Exposure-levels Pathogenesis Pulmonary-system-disorders Respiratory-system-disorders
• ISSN:
  0091-6749
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; Connecticut ; OSHA Region 1 ; OSHA Region 3 ; OSHA Region 9 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  104
• Issue:
  4
• NIOSHTIC Number:
  nn:20030132
• Citation:
  J Allergy Clin Immunol 1999 Oct; 104(4 Pt 1):747-754
• Contact Point Address:
  Division of Pulmonary Disease and Critical Care Medicine, University of Vermont College of Medicine, Burlington, VT 05405
• Federal Fiscal Year:
  2000
• NORA Priority Area:
  Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
• Performing Organization:
  Yale University, New Haven, Connecticut
• Peer Reviewed:
  True
• Part Number:
  1
• Start Date:
  19970901
• Source Full Name:
  Journal of Allergy and Clinical Immunology
• End Date:
  20020831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4435b260cbbdcf438cbdae27ef851c153dd186fcb985f7076b3945602bd5169c1ce0ce992ebedcfa2ba92ed7fd01ceb545d79ab346c0b8f80eaaa147b8fe4fbe
• Download URL:
  https://stacks.cdc.gov/view/cdc/190949/cdc_190949_DS1.pdf
• File Type:
  [PDF - 491.71 KB ]