Benzene Metabolism by the Isolated Perfused Lung

Details

• Personal Author:
  Carlson GP ; Powley MW
• Description:
  Benzene is an occupational hazard and environmental toxicant whose toxic effects are dependent on its metabolism by cytochrome P-450. Most physiologically based pharmacokinetic models assume that benzene is metabolized only in the liver. They may not be completely accurate in predicting metabolism, especially following inhalation exposure, if metabolism by the lung is important. In the current study, the metabolizing capability of the lung was examined in an in vivo simulation using the isolated perfused lung. Lungs from the rabbit, rat, and mouse were used to mimic benzene metabolism following exposure via the pulmonary vasculature. With the isolated perfused mouse lung, three concentrations (55 microM, 120 microM, and 200 microM) were used to evaluate concentration dependence. To evaluate the ability of the lung to metabolize inhaled benzene, the isolated perfused mouse lung was exposed to benzene (approximately 175 ppm) via the trachea. Benzene was metabolized in all species, with phenol being the major metabolite. Phenylsulfate was also detected in perfusate from rabbits and mice but at much lower levels. Benzene metabolism was concentration dependent in mice. The ability of the lung to metabolize benzene during inhalation exposure was demonstrated in the isolated perfused mouse lung. These results demonstrate that the lung can metabolize benzene in an in vivo simulation when exposed via the pulmonary vasculature or via inhalation. [Description provided by NIOSH]
• Subjects:
  Animals Benzene Energy Metabolism Environmental Exposure Environmental Pollution Hazardous Substances Laboratories Liver Lung Lung Diseases Occupational Exposure Occupational Health Respiratory System Respiratory Tract Diseases Safety Toxicology

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• Keywords:
  Animal-studies Benzenes Environmental-hazards In-vivo-studies Inhalation-studies Laboratory-animals Liver-disorders Lung-disorders Metabolism Models Occupational-hazards Pulmonary-system-disorders Simulation-methods Toxic-effects Toxins

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• ISSN:
  0895-8378
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Indiana ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  14
• Issue:
  6
• NIOSHTIC Number:
  nn:20031160
• Citation:
  Inhal Toxicol 2002 Jun; 14(6):569-584
• Email:
  frank@purdue.edu
• CAS Registry Number:
  Benzene (CAS RN 71-43-2)
• Federal Fiscal Year:
  2002
• Performing Organization:
  Purdue University, School of Health Sciences, West Lafayette, Indiana
• Peer Reviewed:
  True
• Start Date:
  20010701
• Source Full Name:
  Inhalation Toxicology
• End Date:
  20270630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b1001e59f1a3657cac16a1fbbe0a48a06218e5fcf7810338832e2c3c102be64d5c99e51572278dd3da38bd246db3854ba1f037c041747e9dceee8e7ece96971f
• Download URL:
  https://stacks.cdc.gov/view/cdc/191495/cdc_191495_DS1.pdf
• File Type:
  [PDF - 368.80 KB ]