Tape-Strip Sampling for Measuring Dermal Exposure to 1,6-Hexamethylene Diisocyanate

Details

• Personal Author:
  Ball LM ; Fent, Kenneth W. ; Gold, Avram ; Jayaraj K ; Nylander-French LA
• Description:
  This study describes the development and evaluation of a method for sampling layers of the stratum corneum for the quantitation of dermal exposure to 1,6-hexamethylene diisocyanate (HDI). HDI deposited on skin was collected by the removal of stratum corneum with adhesive tape, derivatized with 1-(2-methoxyphenyl)piperazine, and quantitated as the urea derivative (HDIU) by liquid chromatography-mass spectrometry (LC-MS). This LC-MS method was tested by analyzing tape spiked with HDI-containing products, then applied to tape samples collected from the skin of an auto-body shop worker exposed to polyurethane paint aerosols. The limits of detection and quantitation were 20 and 50 fmol per injection, respectively. The recovery of HDI from the tape was 99.3% [95% confidence interval (95% CI) 97.1-102]. HDIU was stable at -40 degrees C, degrading by 0.28% (95% CI 0.10-0.46) per day. Quantifiable amounts of HDI were observed in 42.6% of the first three successive tape-strip samples collected from 36 different sites on the skin of the worker. The amount of HDI recovered from the collection sites on skin, measured by summing the levels collected with three successive tape-strips, ranged from nondetectable to 1874 pmol. This study demonstrates that HDI on skin can be collected with tape-strips and quantified at occupational levels using LC-MS. [Description provided by NIOSH]
• Subjects:
  Aerosols Automobiles Environmental Monitoring Mass Spectrometry Occupational Exposure Occupational Health Safety Skin
• Keywords:
  Aerosol-particles Author Keywords: Exposure Assessment Automobile-repair-shops Automotive-industry Exposure-assessment Mass-spectrometry Occupational-exposure Sampling Sampling-methods Sampling Method Skin-exposure Skin Exposure Tape-stripping Worker-health Workers

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• ISSN:
  0355-3140
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  225-240
• Volume:
  32
• Issue:
  3
• NIOSHTIC Number:
  nn:20031151
• Citation:
  Scand J Work Environ Health 2006 Jun; 32(3):225-240
• Contact Point Address:
  Department of Environmental Sciences and Engineering, School of Public Health, The University of North Carolina at Chapel Hill, Rosenau Hall CB #7431, Chapel Hill, NC 27599-7431, USA
• CAS Registry Number:
  Hexamethylene diisocyanate (CAS RN 822-06-0)
• Federal Fiscal Year:
  2006
• Performing Organization:
  University of North Carolina, Chapel Hill
• Peer Reviewed:
  True
• Start Date:
  20030801
• Source Full Name:
  Scandinavian Journal of Work, Environment & Health
• End Date:
  20070731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:68978c5f3340a7d910a18e99ee11b8819ae0ec9d6877bda2cabae65a4857e31acb20f7d8c2974826bd8b0e023fba187ee1c007ac99d5cd5b0d29752ce5b6dbe6
• Download URL:
  https://stacks.cdc.gov/view/cdc/191488/cdc_191488_DS1.pdf
• File Type:
  [PDF - 131.22 KB ]