Bioaerosol Mass Spectrometry for Rapid Detection of Individual Airborne Mycobacterium tuberculosis H37Ra Particles

Details

• Personal Author:
  Fergenson DP ; Frank M ; Gard EE ; Horn J ; Pitesky M ; Schafer, Millie P. ; Tobias HJ
• Description:
  Single-particle laser desorption/ionization time-of-flight mass spectrometry, in the form of bioaerosol mass spectrometry (BAMS), was evaluated as a rapid detector for individual airborne, micron-sized, Mycobacterium tuberculosis H37Ra particles, comprised of a single cell or a small number of clumped cells. The BAMS mass spectral signatures for aerosolized M. tuberculosis H37Ra particles were found to be distinct from M. smegmatis, Bacillus atrophaeus, and B. cereus particles, using a distinct biomarker. This is the first time a potentially unique biomarker was measured in M. tuberculosis H37Ra on a single-cell level. In addition, M. tuberculosis H37Ra and M. smegmatis were aerosolized into a bioaerosol chamber and were sampled and analyzed using BAMS, an aerodynamic particle sizer, a viable Anderson six-stage sampler, and filter cassette samplers that permitted direct counts of cells. In a background-free environment, BAMS was able to sample and detect M. tuberculosis H37Ra at airborne concentrations of >1 M. tuberculosis H37Ra-containing particles/liter of air in 20 min as determined by direct counts of filter cassette-sampled particles, and concentrations of >40 M. tuberculosis H37Ra CFU/liter of air in 1 min as determined by using viable Andersen six-stage samplers. This is a first step toward the development of a rapid, stand-alone airborne M. tuberculosis particle detector for the direct detection of M. tuberculosis bioaerosols generated by an infectious patient. Additional instrumental development is currently under way to make BAMS useful in realistic environmental and respiratory particle backgrounds expected in tuberculosis diagnostic scenarios. [Description provided by NIOSH]
• Subjects:
  Aerosols Air Pollutants, Occupational Biomarkers Mass Spectrometry Occupational Health Particulate Matter Safety
• Keywords:
  Aerosol-particles Airborne-particles Mass-spectrometry Sampling Sampling-methods
• ISSN:
  0099-2240
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  California ; Ohio ; OSHA Region 5 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  71
• Issue:
  10
• NIOSHTIC Number:
  nn:20028673
• Citation:
  Appl Environ Microbiol 2005 Oct; 71(10):6086-6095
• Contact Point Address:
  L-452 Lawrence Livermore National Laboratory, 7000 East Ave, Livermore, CA 94550
• Email:
  gard2@llnl.gov
• Federal Fiscal Year:
  2006
• Peer Reviewed:
  True
• Source Full Name:
  Applied and Environmental Microbiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:51d6fdd3bf7bce85dd5340fc21250320ce8822961382e8b007664de33565c6a1c4923f7a48e50534722703ec9005e759da985af15782147dc26bd6aad0407d8b
• Download URL:
  https://stacks.cdc.gov/view/cdc/189879/cdc_189879_DS1.pdf
• File Type:
  [PDF - 432.42 KB ]