Detection of Allergens from Alternaria alternata by Gold-Conjugated Anti-Human IgE and Field Emission Scanning Electron Microscopy

Details

• Personal Author:
  Eduard W ; Green, Brett J. ; Romeo TC ; Sercombe JK ; Tovey ER
• Description:
  Fungal allergens are present in viable and non-viable conidia, hyphae and fungal fragments. It has been shown that large quantities of allergen are released from conidia during germination. We used a gold immunolabelling technique and field emission scanning electron microscopy to examine the allergen release from Alternaria alternata conidia. Immunolabelling was associated with the hyphal tip and amorphous matter associated with the emerging hyphae. Non-specific antibody controls showed no labelling associated with germinating fungi. This suggests that material released from hyphae may be an additional source of fungal allergens. [Description provided by NIOSH]
• Subjects:
  Fungi Fungicide, Industrial Hypersensitivity Immune System Microbiology Occupational Health Safety
• Keywords:
  Allergens Fungal-diseases Fungal-infections Fungicides Immunology Microorganisms Microscopy
• ISSN:
  0022-1759
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  167-170
• Volume:
  316
• Issue:
  1
• NIOSHTIC Number:
  nn:20031143
• Citation:
  J Immunol Methods 2006 Oct; 316(1-2):167-170
• Contact Point Address:
  PO Box 8149, NO-0033 Oslo, Norway
• Email:
  wijnand.eduard@stami.no
• Federal Fiscal Year:
  2007
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Immunological Methods
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7f37cfac9d61cf82fc8f2f61937cb25bd40ec419ca7265e86f41e5424b76da1f0d165cc1ba28a23d11da100e2d7bf5037cde9d088cab7c323bbfd574f0ede9ce
• Download URL:
  https://stacks.cdc.gov/view/cdc/191485/cdc_191485_DS1.pdf
• File Type:
  [PDF - 546.75 KB ]