Hemoglobin Potentiates the Generation of Reactive Oxygen Species by Alveolar Macrophages

Details

• Personal Author:
  Bowman L ; Huffman LJ ; Miles PR ; Shi X
• Description:
  The objectives of the present investigation were to study the effects of hemoglobin on tree radical production by alveolar macrophages. Alveolar macrophages were harvested from anesthetized male rats. Free radical production by alveolar macrophages was assessed by measuring integrated luminol-enhanced chemiluminescence responses (cpmx10^5/.5x10^6 cells) and electron-spin resonance profiles under basal and activated conditions. A soluble phorbol ester (PMA; 12-0- tetradecanoylphorboI-13-acetate) was used to initiate free radical production by alveolar macrphages. Hemoglobin (0.1-1.00 microM) significantly enhanced PMA-stimulated chemiluminescence from alveolar macrophages in a dose-related manner. The possibility that hemoglobin may act as a biological Fenton reagent to promote enhanced free radical responses by alveolar macrophages was explored next. Addition of either an iron chelator, the removal of hydrogen peroxide or superoxide anions, or the presence of hydroxyl radical scavengers significantly decreased the effect of hemoglobin. These findings suggest that hemoglobin enhances free radical production by alveolar macrophages by acting as a biological catalyst and that the pronounced increase in reactive species generation is the overall result of an iron-catalyzed HaberWeiss reaction. Thus, alveolar macrophages may be a cell type which contributes to the potentiation of lung damage when hemoglobin is present. [Description provided by NIOSH]
• Subjects:
  Animals Environmental Monitoring Hematologic Diseases Laboratories Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Animal-studies Blood-disorders Dose-response Exposure-assessment Exposure-levels Inhalation-studies Laboratory-animals Lung-disorders Pulmonary-system-disorders Respiratory-system-disorders
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  District of Columbia ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  13
• Issue:
  4
• NIOSHTIC Number:
  nn:20030640
• Citation:
  FASEB J 1999 Mar; 13(4)(Pt 1):A171
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  False
• Part Number:
  1
• Source Full Name:
  The FASEB Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e32430624ac695ff1adefccd7265ff4ec2a5a980ea32b820f96eee713a45d1a23909516f14d27150ecec750e6850d3b72c9c0294f8d1075c98c25088ac351e14
• Download URL:
  https://stacks.cdc.gov/view/cdc/191182/cdc_191182_DS1.pdf
• File Type:
  [PDF - 336.99 KB ]