A Rapid, Sensitive, and Specific Immunochromatographic Lateral Flow Assay for Anthrax Protective Antigen Immunity Status: Development and Evaluation

Details

• Personal Author:
  Biagini, Raymond E. ; MacKenzie, Barbara A. ; Robertson SR ; Sammons DL ; Smith JP ; Snawder, John E. ; Striley CAF
• Description:
  Evidence from animals suggests that anti-protective antigen (PA) IgG from vaccination with Anthrax Vaccine Adsorbed (AVA) is protective against B. anthracis infection. Measurement of anti-PA IgG in human sera can be performed using either Enzyme-Linked Immunosorbent Assay (ELISA) or Fluorescent Covalent Microsphere Immunoassay (FCMIA, Clin Lab Diagnostic Immunol 11 :50-55, 2004). Both these methods are laboratory based. We describe the development of a rapid lateral flow immunochromatograhic assay (LFIA) test kit for the measurement of anti-PA IgG in serum or whole blood (50 ul sample) using colloidal gold nanoparticles as the detection reagent and an internal control. Using sera from 19 AVA vaccinees (anti-PA IgG range, 2.4 - 267 ug/ml), 10 controls and PA-supplemented whole blood, we demonstrated the LFIA had a sensitivity of -2 ug/ml anti-PA IgG in sera and -14 ug/ml anti-PA IgG in whole blood. Pre-adsorption of sera with PA yielded negative anti-PA LFIAs. Internal controls were positive for all tests. The diagnostic sensitivity and specificity of the assay (for human sera) were 100% using FCMIA anti-PA IgG as standard. This kit has utility for determining the immune status of individuals (military personnel, decontamination workers) vaccinated with AVA or possibly exposed to sub-clinical levels of B. anthracis. [Description provided by NIOSH]
• Subjects:
  Biological Warfare Agents Occupational Health Risk Assessment Safety Toxicology
• Keywords:
  Biological-warfare-agents Biological-weapons Decontamination Nanotechnology Risk-analysis
• Document Type:
  Text
• Funding:
  Interagency Agreement
• Genre:
  Abstract or Summary
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  22
• NIOSHTIC Number:
  nn:20030188
• Citation:
  2005 Toxicology and Risk Assessment Conference, April 25-28, 2005, Fairborn, Ohio. 2005 Apr; :22
• Federal Fiscal Year:
  2005
• Peer Reviewed:
  False
• Source Full Name:
  2005 Toxicology and Risk Assessment Conference, April 25-28, 2005, Fairborn, Ohio
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a96e6100b5c5bda24f9e49262b56762c941296ba24c49e4e38835aa1ad7df863b6cbe59941ba482fa93404ff30c5c7d858b3f281c7001a71d890dd271f278050
• Download URL:
  https://stacks.cdc.gov/view/cdc/190963/cdc_190963_DS1.pdf
• File Type:
  [PDF - 163.04 KB ]