Multiplexed Measurement of Specific IgGs to Five CDC Select Bioterrorism Agents in Serum

Details

• Personal Author:
  Biagini, Raymond E. ; MacKenzie, Barbara A. ; Quinn CP ; Robertson SR ; Sammons DL ; Smith JP ; Snawder, John E. ; Striley CAF
• Description:
  The Centers for Disease Control and Prevention has classified several agents which may be used in a bioterrorism attack as "select agents". They are classified as such based on ease of dissemination, mortality/morbidity rate, and potential for social disruption. A subset of these agents include Bacillus anthracis (Ba), Yersinia pestis (Yp), Francisella tularensis (Ft) , Ricin toxin (RT) and Staphylococcal enterotoxin B (SEB). Exposure/infection with these agents has been shown to cause the production of specific serum IgG antibodies. Comparison of pre- and post-incident IgG antibody levels has been shown to be a useful method to biologically monitor decontamination and clean-up workers for potential exposure to bioterrorism agents (Occ Environ Med 61: 703-708, 2004). We describe a fluorescent covalent microsphere immunoassay (FCMIA) to measure specific IgG antibodies to Ba (protective antigen [PA] and lethal factor [LF]), Yp (F1 and V antigens), Ft, RT and SEB simultaneously in human Ba vaccinee sera which has been fortified with animal select agent specific IgG antibodies. Intra- and interassay coefficients of variation were <15% (N=3). There were no significant differences (P>0.70) in dilution curves when the assays were performed individually vs. multiplexed. When the observed vs. expected dilution curves were compared, highly linear relationships were observed (mean r2=0.995 + 0.006 [SO]). Finally, the curves yielded linear responses for most analytes upon serum dilution from 1 x102 to 1 x105. These data indicate that multiplexed FCMIA is a method to measure specific IgGs in serum to CDC select agents and may be of value in screening either decontamination workers or the general population for exposures/infections to these agents. [Description provided by NIOSH]
• Subjects:
  Biological Warfare Agents Occupational Health Risk Assessment Safety Toxicology
• Keywords:
  Biological-warfare-agents Biological-weapons Decontamination Risk-analysis
• Document Type:
  Text
• Funding:
  Interagency Agreement
• Genre:
  Abstract or Summary
• Place as Subject:
  Georgia ; Ohio ; OSHA Region 4 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  21
• NIOSHTIC Number:
  nn:20030187
• Citation:
  2005 Toxicology and Risk Assessment Conference, April 25-28, 2005, Fairborn, Ohio. 2005 Apr; :21
• Federal Fiscal Year:
  2005
• Peer Reviewed:
  False
• Source Full Name:
  2005 Toxicology and Risk Assessment Conference, April 25-28, 2005, Fairborn, Ohio
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:59e8a6ea314118721e848d6b9f05cb729961baab61f712e851bf0bb3ca542c72673fc466d7ffbea713071ab8b976e7a9226408405dd06a69bed698bee071b9e0
• Download URL:
  https://stacks.cdc.gov/view/cdc/190962/cdc_190962_DS1.pdf
• File Type:
  [PDF - 177.31 KB ]