Simultaneous Measurement of Specific Serum IgG Responses to Five Select Agents

Details

• Personal Author:
  Biagini, Raymond E. ; MacKenzie, Barbara A. ; Quinn CP ; Robertson SA ; Sammons DL ; Smith JP ; Snawder, John E. ; Striley CAF
• Description:
  Select Agents are defined by CDC and the USDA Animal and Plant Health Inspection Service (APHIS) as biological agents or toxins deemed a threat to public, animal, or plant health, or to animal or plant products. They are classified based on their ease of dissemination, mortality/morbidity rate, and potential for social disruption. A subset of these agents includes Bacillus anthracis, Yersinia pestis, Francisella tularensis, ricin toxin (RT), and staphylococcal enterotoxin B (SEB). Infection or intoxication with these agents has been shown to elicit an antigen specific serum IgG response. We describe a fluorescent covalent microsphere immunoassay (FCMIA) for measurement of specific IgG antibodies to seven different antigens from five different select agents: B. anthracis [protective antigen (PA) and lethal factor (LF)], Y. pestis (F1 and V antigens), F. tularensis, RT and SEB simultaneously in human B. anthracis vaccinee sera (containing anti-PA and anti-LF IgG) which had been spiked with animal specific IgG antibodies to the other select agents. Inter- and intra-assay coefficients of variation were 6.5% and 13.4%, respectively (N = 4). There were no significant differences (P greater than 0.70) in assay responses when the assays were performed individually versus multiplexed. When the observed versus expected interpolated concentrations were compared using standards recovery, highly linear relationships were observed (r2 values from 0.981 to 0.999, P less than 0.001). Minimum detectable concentrations (MDCs) ranged from 0.3 ng/ml (anti-Y. pestis F1 IgG) to 300 ng/ml (anti-RT IgG). Linear dynamic ranges spanned from 125 ng/ml for anti-SEB IgG to 1333 ng/ml for anti-Y.pestis F1 IgG. The multiplexed FCMIA is considerably faster and has extremely higher throughput when compared to existing individual enzyme-linked immunosorbent assays (ELISAs) commonly used for measurement of anti-BT IgGs. These data indicate that multiplexed FCMIA is a sensitive, accurate and precise method to simultaneously measure specific IgGs in serum to CDC select agents and may be of value in screening either decontamination workers, the general population or animals for exposures/infections to these agents. [Description provided by NIOSH]
• Subjects:
  Antibodies Antigens Biological Assay Biological Products Biological Warfare Agents Communicable Diseases Hazardous Substances Immune System Occupational Health Safety
• Keywords:
  Antibody-response Bioassays Biological-agents Biological-monitoring Biological-warfare-agents Biological-weapons Decontamination Immunoglobulins Infection-control Infectious-diseases Serological-techniques Toxins

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• Document Type:
  Text
• Funding:
  Interagency Agreement
• Genre:
  Abstract or Summary
• Place as Subject:
  Georgia ; North Carolina ; Ohio ; OSHA Region 4 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1
• NIOSHTIC Number:
  nn:20030183
• Citation:
  Fort Detrick Symposium on Biodefense and Infectious Diseases, April 28-29, 2005, Fort Detrick, Maryland. 2005 Apr; :1
• Contact Point Address:
  National Institute for Occupational Safety and Health, Division of Applied Research and Technology, Biomonitoring and Health Assessment Branch, 4676 Columbia Parkway, Cincinnati, OH 45226
• Email:
  reb4@cdc.gov
• CAS Registry Number:
  Divinylbenzene (CAS RN 1321-74-0) ; Methacrylic acid (CAS RN 79-41-4) ; Polystyrene (CAS RN 9003-53-6) ; Ricins (CAS RN 9009-86-3)
• Federal Fiscal Year:
  2005
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  False
• Source Full Name:
  Fort Detrick Symposium on Biodefense and Infectious Diseases, April 28-29, 2005, Fort Detrick, Maryland
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3c82413101e2dcb6b6013318ea67ae2925d9c7f723ac9840c071fad29cd78f96414a0c3c1b87d43426749149535cedc63ba17c485fb9decc9887ceeed82fa906
• Download URL:
  https://stacks.cdc.gov/view/cdc/190961/cdc_190961_DS1.pdf
• File Type:
  [PDF - 300.10 KB ]