Localization of Species-Specific Antibody Binding Sites to Stachybotrys chartarum Using the Halogen Immunoassay

Details

• Personal Author:
  Beezhold, Donald H. ; Blachere F ; Green, Brett J. ; Millecchia L ; Schmechel D
• Description:
  The potential health risks associated with exposure to Stachybotrys charta rum are currently the subject of intense research, however species-specific detection of the fungus using immunoassays is not available. The purpose of this study was to use the Halogen Inununoassay (HIA) to characterize the antibody binding sites of a recently developed S. chartarum monoclonal antibody (mAb 9B4), in pre- and post-germinated conidia. Antigen expression was detected using mAb 9B4 in the IDA. Aerosolized conidia and fungal fragments were collected onto protein binding membranes direct from sporulating in vitro culture plates. To germinate conidia, samples were incubated in high humidity at room temperature for 48 hours. Ungerminated and germinated samples were then laminated with an adhesive coverslip and immunostained by the HIA. The samples were examined by confocal and light microscopy and positive (haloed) particles were expressed as percentages of total spores. The localization of specific mAb 9B4 binding sites in all pre- and post- germinated samples was primarily restricted to the conidiogenous phialides and around phialoconidia. Post-germination, the proportion of phialides releasing antigen remained unchanged (95.3 +/- 1.5) from ungerminated treatments (96.9 +/- 1.1; P=0.384); however there was a significant increase (P<0.0001) in the proportion of germinated spores expressing antigen (53.3 +/- 2.9) compared to ungerminated spores (6.6 +/- 1.1). No immunostaining was detected in either hyphal fragments or hyphae from germinated spores in pre- and post-germinated treatments. This study further supports that mAb 9B4 recognizes a sporulation specific antigen localized in the phialides and phialoconidia that can be used for Stachybotrys-specific immunoassays. [Description provided by NIOSH]
• Subjects:
  Aerosols Antibodies Fungi Fungicide, Industrial Hazardous Substances Humidity Hypersensitivity Immune System Occupational Health Risk Assessment Risk Factors Safety Temperature

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• Keywords:
  Aerosol-particles Allergens Allergies Antibody-response Fungicides Health-hazards Immunology In-vitro-studies Risk-analysis Risk-factors Sampling Temperature-effects

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• ISSN:
  0091-6749
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  17
• Issue:
  2
• NIOSHTIC Number:
  nn:20029977
• Citation:
  J Allergy Clin Immunol 2006 Feb; 117(2)(Suppl 1):S31
• Federal Fiscal Year:
  2006
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  False
• Source Full Name:
  Journal of Allergy and Clinical Immunology
• Supplement:
  1
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:75e39e8b45f61538e744360c80b9afd853ffadc64a0c935163a04821a2a04d2e6c8218ed7e6601e0e3ac578f4505ba7601c37466dfb2a9467dae3b199c115b86
• Download URL:
  https://stacks.cdc.gov/view/cdc/190840/cdc_190840_DS1.pdf
• File Type:
  [PDF - 184.83 KB ]