Application of Taqman Chemistry for Allelic Discrimination

Details

• Personal Author:
  Johnson VJ
• Description:
  Taqman chemistry has been successfully adapted for use as a tool to discriminate allelic variation in human study populations. This technique has been validated against a number of well-accepted techniques including PCR-RFLP, sequencing, and allele-specific PCR. The Taqman assays are now being used as the basis for validation of newer genotyping strategies indicating its growing acceptance in the scientific literature. These assays are amenable to the high-throughput genotyping demands of the post-human genome sequencing era and are associated with less cost and technical error compared to more traditional approaches. The application of microfluidics and nanoliter volumes will greatly further the high-throughput/low cost attributes of this genotyping platform. [Description provided by NIOSH]
• Subjects:
  Biological Assay Biomarkers Chemistry Chemistry, Analytic DNA Genetics Occupational Health Safety
• Keywords:
  Analytical-chemistry Analytical-processes Bioassays DNA-damage Genotoxicity
• ISBN:
  0974876550
• Publisher:
  DNA Press
• Document Type:
  Text
• Genre:
  Book
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  237-254
• NIOSHTIC Number:
  nn:20029789
• Citation:
  Genetic variance detection: technologies for pharmacogenomics. Hecker KH, ed. Eagleville, PA: DNA Press, 2005 Jan; :237-254
• Contact Point Address:
  Victor J. Johnson, Toxicology and Molecular Biology Branch, Health Effects Laboratory division, NIOSH/CDC, 1095 Willowdale Road, M/S 3014, Morgantown, WV 26505-2888
• Email:
  VJohnson3@cdc.gov
• Editor(s):
  Hecker KH
• Federal Fiscal Year:
  2005
• NORA Priority Area:
  Disease and Injury: Asthma and Chronic Obstructive Pulmonary Disease
• Peer Reviewed:
  False
• Source Full Name:
  Genetic variance detection: technologies for pharmacogenomics
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4d2eb99a206c13932f1d49d30256f5c02e1295b1e1b71d4e372dd68c13273540ebcec0a9efe80bff0c295d1e1d336efe865092b006569d43dca6966b592fc3bf
• Download URL:
  https://stacks.cdc.gov/view/cdc/190699/cdc_190699_DS1.pdf
• File Type:
  [PDF - 2.19 MB ]