Cell Proliferation in Over-Loaded Rat Soleus Muscles: Myofiber Splitting vs Myogenesis

Details

• Personal Author:
  Smith CA ; Stauber WT
• Description:
  Injury to skeletal muscle has been associated with proliferation and migration of cells into regions with damaged myofibers. Many of these cells have been presumed to be inflammatory cells or phagocytes. Using two types of functional overload we have produced damage to myofibers which mimics overuse syndromes. By applying specific antibodies and using double-labeling techniques, we have identified macrophages, degenerating myofibers, fibroblasts, split myofibers and new myobalsts. Myoblasts can be visualized using desmin and dystrophin or desmin and vimentin. Fibroblasts stain only for vimentin. The location of central nuclei and intracellular deposition of vimentin, desmin and fibronectin appear to be related to myofiber splitting. Some myofibers which appear to have split contain different myosin isoforms whereas others are homogeneous. [Description provided by NIOSH]
• Subjects:
  Animals Laboratories Musculoskeletal Diseases Musculoskeletal System Occupational Health Safety
• Keywords:
  Animal Studies Cell Migration Injuries Laboratory Animals Muscles Musculoskeletal System Disorders Phagocytes Skeletal Disorders Skeletal System
• ISSN:
  0031-9376
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  39
• Issue:
  5
• NIOSHTIC Number:
  nn:20029755
• Citation:
  Physiologist 1996 Oct; 39(5):A92
• Contact Point Address:
  Departments of Physiology and Neurology, Robert C. Byrd Health Sciences Center, West Virginia University, PO Box 9229, Morgantown, WV 26506-9229
• Federal Fiscal Year:
  1997
• Performing Organization:
  West Virginia University, Morgantown, West Virginia
• Peer Reviewed:
  False
• Start Date:
  19920101
• Source Full Name:
  The Physiologist
• End Date:
  20050531
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:897307f9e4ee62a8c45fbe8a16096e30570f163b1349cd3523a8180a62fc90ed0b60e92ea4df37738806f2e1c615a2be27df238916eb36e51700c3be6e118db5
• Download URL:
  https://stacks.cdc.gov/view/cdc/190672/cdc_190672_DS1.pdf
• File Type:
  [PDF - 231.96 KB ]