Diisocyanate Antigen-Stimulated Monocyte Chemoattractant Protein-1 Synthesis Has Greater Test Efficiency than Specific Antibodies for Identification of Diisocyanate Asthma

Details

• Personal Author:
  Bernstein DI ; Boulet LP ; Cartier A ; Cote J ; L'Archeveque J ; Lummus Z ; Malo JL ; Milot J ; Trudeau C ; Wanner M
• Description:
  We previously reported that diisocyanate-human serum albumin (DIISO-HSA) stimulated production of monocyte chemoattractant protein-1 (MCP-1) by peripheral blood mononuclear cells is significantly associated with a clinical diagnosis of diisocyanate asthma (DA). Others have reported that antibodies for DIISO-HSA are specific but insensitive markers of DA. This study was performed to evaluate test characteristics of the in vitro MCP-1 assay compared with DIISO-HSA-specific immunoglobulin (Ig) G and IgE in identifying workers with DA. MCP-1 was quantitated in peripheral blood mononuclear cell supernatants 48 hours after incubation with DIISO-HSA antigens. Assay results were compared with outcomes of specific inhalation challenge (SIC) testing. Nineteen of 54 (35%) workers assayed for antibodies and MCP-1 stimulation had SIC-confirmed DA. Mean MCP-1 produced by SIC-positive workers was greater than SIC-negative workers (p < or = 0.001). Diagnostic sensitivity, specificity, and test efficiency for specific IgG were 47%, 74%, and 65%, respectively, and for specific IgE were 21%, 89%, and 65%, respectively. Sensitivity, specificity, and test efficiency of the MCP-1 test were 79%, 91%, and 87%, respectively. This study indicates that the MCP-1 stimulation assay has greater sensitivity and specificity than the specific antibody assays in correctly identifying DA. [Description provided by NIOSH]
• Subjects:
  Asthma Asthma, Occupational Biological Factors Biomarkers Occupational Health Respiratory Tract Diseases Safety
• Keywords:
  Antibody Author Keywords: Occupational Asthma Biological-factors Bronchial-asthma Diisocyanate Genotoxic-effects Humans MCP-1 Pulmonary-system-disorders Respiratory-system-disorders Worker-health Workers

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• ISSN:
  1073-449X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  445-450
• Volume:
  166
• Issue:
  4
• NIOSHTIC Number:
  nn:20029474
• Citation:
  Am J Respir Crit Care Med 2002 Aug; 166(4):445-450
• Contact Point Address:
  Department of Internal Medicine, Division of Immunology, College of Medicine, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, OH 45267-0563
• Email:
  bernstdd@email.uc.edu
• Federal Fiscal Year:
  2002
• Performing Organization:
  University of Cincinnati, Cincinnati, OH
• Peer Reviewed:
  True
• Start Date:
  19990930
• Source Full Name:
  American Journal of Respiratory and Critical Care Medicine
• End Date:
  20030929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a5f20ebe0917331e546802e7ee187118b43d80f0d4ccdc91f85457d1b6622b68464836751f3171e9cd276f341453afc384398c79f9ce3991b62831a3ec449fa3
• Download URL:
  https://stacks.cdc.gov/view/cdc/190440/cdc_190440_DS1.pdf
• File Type:
  [PDF - 116.82 KB ]