Increased Levels of Sperm Ubiquitin Correlate with Semen Quality in Men from an Andrology Laboratory Clinic Population

Details

• Personal Author:
  Hauser R ; Sutovsky M ; Sutovsky P
• Description:
  BACKGROUND: Ubiquitin, a house-keeping protein that marks other proteins for proteasomal degradation, tags defective sperm during epididymal passage. To establish ubiquitin as a biomarker of human infertility, the present study examines the relationships between sperm ubiquitin content and clinical semen parameters among men from an infertility clinic population with varied aetiologies. METHODS: Anti-ubiquitin immunoreactivity was measured by flow cytometric sperm-ubiquitin tag immunoassay (SUTI) in sperm samples of 28 infertility patients and 15 fertile donors. Semen analyses were performed by computer-assisted semen analysis and World Health Organization morphology. RESULTS: Median values of ubiquitin-induced fluorescence had a strong negative correlation with sperm count (r = -0.63, P = 0.0003) and a positive correlation with % abnormal morphology (r = 0.55, P = 0.01). Infertility patients (n = 28) had significantly higher levels of sperm ubiquitin. Out of 28 patients, six reported possible occupational exposures to solvents, three were current smokers and six were ex-smokers. Within the patient group, men with known male factor infertility, those with self-reported occupational exposure to solvents and current smokers had the highest sperm ubiquitin levels. When men with jobs involving potential occupational exposure to solvents were combined with current smokers, the highest correlations were found between sperm ubiquitin and motility (r = -0.74), count (r = -0.82) and % sperm abnormalities (r = 0.73). CONCLUSIONS: Increased sperm ubiquitin was inversely associated with sperm count, motility and % normal morphology, supporting the use of ubiquitin as a biomarker of human semen quality. SUTI assay confirmed poor semen quality in all men with poor clinical semen parameters, but also was high in some patients with seemingly good clinical semen parameters. Occupational exposure to solvents and smoking may have contributed to high levels of sperm ubiquitin in some of these patients. [Description provided by NIOSH]
• Subjects:
  Occupational Health Safety Spermatogenesis Urogenital Diseases Urogenital System
• Keywords:
  Fertility Mammalian-cells Reproductive-system Reproductive-system-disorders Spermatozoa
• ISSN:
  0268-1161
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Missouri ; Oregon ; OSHA Region 10 ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  19
• Issue:
  3
• NIOSHTIC Number:
  nn:20029413
• Citation:
  Hum Reprod 2004 Mar; 19(3):628-638
• Contact Point Address:
  Department of Animal Science, University of Missouri-Columbia, 65211-5300
• Email:
  SutovskyP@missouri.edu
• Federal Fiscal Year:
  2004
• Performing Organization:
  University of Missouri, Columbia, Missouri
• Peer Reviewed:
  True
• Start Date:
  20000930
• Source Full Name:
  Human Reproduction
• End Date:
  20020929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:897ec1cb56c3e7e0dab459e7dec258ab6774310c33837ff8dde953c3f67816948592e94dab69ff0c6e8b60d6712fe9a220d4a29dd528f06b5169f4aba231c7ce
• Download URL:
  https://stacks.cdc.gov/view/cdc/190398/cdc_190398_DS1.pdf
• File Type:
  [PDF - 347.90 KB ]