Role of cyclinD1 and CDK4 in the Carcinogenesis Induced by Silica

Details

• Personal Author:
  Liu BC ; Shi XL ; Xu M ; Yan KX ; You BR
• Description:
  To study the role of cyclinD1 and CDK4 in malignant transformation of human fetal lung diploid fibroblast cell line (2BS) induced by silica. Recombination vectors with sense and antisense pXJ41-cyclinDl and pXJ41-CDK4 were constructed, and then transfected into the malignant transformed cells induced by silica, respectively. At the same time, pXJ41-neo was used as the control. During the progress of the malignant transformation of 2BS cells induced by silica, cyclinD1 and CDK4 were overexpressed. Antisense RNA suppressed cyclinD1 and CDK4 gene expression in the antisense pXJ41-cyclinDl and pXJ41-CDK4 transfected cells. Antisense RNA led to cell cycle arrest, resulting in lengthened G1 phase (the percentages of cells in the G1 phase changed from 45.1% to 52.7% and 58.0% for cyclinD1 and CDK4 transfected cells, respectively), and eventually attenuated the increase of the proliferation of malignant transformed cells induced by silica. Compared with malignant transformed cells induced by silica, cells transfected with antisense pXJ41-cyclinD1 and pXJ41-CDK4 showed obviously reduced growth rates. On the 8th day, the suppression rates were 58.69 and 77.43% (the growth rate of malignant transformed cells induced by silica was 100%), doubling time changed from 21.0 h to 31.4 h and 21.0 h to 42.7 h, respectively, the growth capacities on soft agar of cells transfected by antisense pXJ41-cyclinD1 and pXJ41-CDK4 decreased obviously. CyclinD1 and CDK4 play an important role in maintaining transformed phenotype of the cancer cells. [Description provided by NIOSH]
• Subjects:
  Aluminum Silicates Carcinogens Free Radicals Lung Lung Diseases Neoplasms Occupational Health Respiratory System Respiratory Tract Diseases Safety Silicon Dioxide

  More +
• Keywords:
  Antisense RNA Author Keywords: CyclinD1 Breast-cancer Cancer Carcinogenesis Carcinogenicity CDK4 Cell-transformation Free-radicals Lung-cancer Silica Silica-dusts Silicates

  More +
• ISSN:
  0895-3988
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  286-296
• Volume:
  18
• Issue:
  5
• NIOSHTIC Number:
  nn:20028773
• Citation:
  Biomed Environ Sci 2005 Oct; 18(5):286-296
• Contact Point Address:
  Professor Bing-Ci LIU, National Institute of Occupational Health and Poisons Control, Chinese Center for Disease Control and Prevention, 29 Nanwei Road, Beijing 100050, China
• Email:
  bcliu@263.net
• Federal Fiscal Year:
  2006
• NORA Priority Area:
  Research Tools and Approaches: Cancer Research Methods
• Peer Reviewed:
  True
• Source Full Name:
  Biomedical and Environmental Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:43b4b395eba4a883616363e5868035fc5854b65accd94dc6fcf1980810a3812d080444ef9d461b24b964c84d7373258cf18cf054a043db7b37fb5f5e90e322ab
• Download URL:
  https://stacks.cdc.gov/view/cdc/189911/cdc_189911_DS1.pdf
• File Type:
  [PDF - 1.84 MB ]