Changes in F-Actin Organization Induced by Hard Metal Particle Exposure in Rat Pulmonary Epithelial Cells As Observed by Laser Scanning Confocal Microscopy

Details

• Personal Author:
  Antonini JM ; Millecchia LL ; Rao KMK ; Roberts, Jennifer R. ; Starks K ; Yang HM
• Description:
  Hard metal is an alloy of tungsten carbide and cobalt along with other components such as chromium carbide, molybdenum carbide, tantalum carbide, and nickel. Chronic exposure to hard metal particles by inhalation causes alveolitis leading to interstitial fibrosis, the pathogenesis of which is still undefined. The initial inflammatory response includes a change in epithelial cell permeability barrier function (1) which has been shown to be regulated by the state of assembly and organization of the actin cytoskeletal network (2, 3). Therefore, the objective of this study was to evaluate the effect hard metal particles have on F-actin organization of rat lung epithelial cells in an in vitro culture system. [Description provided by NIOSH]
• Subjects:
  Animals Dust Fibrosis Laboratories Lung Metals Metals, Heavy Occupational Health Poisoning Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Animal-studies Exposure-levels Heavy-metal-poisoning Heavy-metals Inhalation-studies Laboratory-animals Lung-cells Lung-fibrosis Metal-compounds Metal-dusts Metal-poisoning Pulmonary-system-disorders Respiratory-system-disorders

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• ISSN:
  1431-9276
• Document Type:
  Text
• Genre:
  Proceedings
• Place as Subject:
  Oregon ; OSHA Region 10 ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  492-493
• Volume:
  5
• NIOSHTIC Number:
  nn:20028083
• Citation:
  Microsc Microanal 1999 Aug; 5(Suppl 2):492-493
• Email:
  jga6@cdc.gov
• CAS Registry Number:
  Cobalt (CAS RN 7440-48-4) ; Molybdenum (CAS RN 7439-98-7) ; Nickel (CAS RN 7440-02-0)
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  False
• Source Full Name:
  Microscopy and Microanalysis
• Supplement:
  2
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:3b89182d1c2ff09d8e5ab5fadc1850e22fd928da48ca5b47bf75a3548ee24eb873dcdc58d46b799a960556dcec5641e11565fbe8f361181dcb20571efa59197d
• Download URL:
  https://stacks.cdc.gov/view/cdc/189765/cdc_189765_DS1.pdf
• File Type:
  [PDF - 475.72 KB ]