Arsenic Stimulates Bladder Epithelial Cell Proliferation

Details

• Personal Author:
  Flood L ; Germolec D ; Luster MI ; Matheson JM ; Simeonova PP ; Toriumi W
• Description:
  Epidemiological studies have demonstrated a strong correlation between increased arsenic concentrations in drinking water and the incidence of bladder cancer in human. Additionally, it has been reported that dirnethylarsinic, one of the metabolites of inorganic arsenicals, promotes nitrosamine induced rat urinary bladder carcinogenesis. We have used in vivo and in vitro models to analyze the effect of inorganic arsenic (As3+) on bladder epithelial cells. Rats treated with arsenic in the drinking water (0.02%) had high levels in the urine of total arsenic represented by DMA, MMA and As3+, and demonstrated an increase in bladder epithelial cell proliferation in 4 weeks. The proliferative changes were documented by HE staining, PCNA immunostaining and electron microscopy. Consistent with the in vivo data, arsenic induced an increase in [3-H) thymidine incorporation in several bladder epithelial cell lines, a marker for DNA synthesis and cell proliferative response. Furthermore, arsenic stimulated the activation of transcription factors associated with modulation of the growth factor expression and control of the cell cycle of bladder epithelial cells, including AP-l. Taken together, these studies suggest that arsenic-induced bladder cancer may be associated with the ability of arsenic to directly stimulate bladder epithelial cell growth. Cell proliferation alone can provide an explanation of carcinogenesis. Arsenic can promote cell proliferation of previously initiated cells or the rapid cell proliferation can be prerequisite for increased mutational rate. [Description provided by NIOSH]
• Subjects:
  Animals Arsenic Carcinogens Hazardous Substances Inorganic Chemicals Laboratories Neoplasms Occupational Health Poisoning Safety Toxicology Urogenital Diseases Urogenital System

  More +
• Keywords:
  Animal-studies Arsenic-compounds Arsenic-poisoning Bladder-cancer Bladder-disease Bladder-disorders Cancer In-vitro-study In-vivo-study Laboratory-animals Toxic-materials Toxins

  More +
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Louisiana ; North Carolina ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 6 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  48
• NIOSHTIC Number:
  nn:20028022
• Citation:
  Toxicologist 1999 Mar; 48(1-S):281
• CAS Registry Number:
  Arsenic (CAS RN 7440-38-2)
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 38th Annual Meeting, March 14-18, 1999, New Orleans, Louisiana
• Supplement:
  1-S
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:71cdd3ed7decee58db49686f3f38ddbfe85f4f7c055db6ed1acb36e86dca3c83c54433136a94e9b0bda8aa10710709fb48f8b71235010282e6afac31f094f9e0
• Download URL:
  https://stacks.cdc.gov/view/cdc/189732/cdc_189732_DS1.pdf
• File Type:
  [PDF - 225.48 KB ]