Toughness of Skin in Arsenic Exposed Mice: Role of GM-CSF and TGFa

Details

• Personal Author:
  Aikawa H ; Fujimoto W ; Germolec DR ; Kayama F ; Luster MI ; Sakabe K ; Yamauchi H ; Yoshida T
• Description:
  Hyperkeratosis on the palms of the hand and soles of the feet are characteristics of arsenic (As) poisoning. However, toughness of skin has not been described as a manifestation of As poisoning. We previously reported that As induces GM-CSF and TGFa mRNA and protein production in human keratinocyte culture. In the present studies sodium arsenite was provided at 1 and 10mg/L to C57B/6n male and female mice via drinking water for 50 days. As concentrations were determined in hair and urine. Pieces of skin were excised from shaved ventral skin by scissors for histpathological and molecular analysis. Increase skin toughness dominantly in male was apparent in exposed groups when skin sections were prepared. Skin sections were prepared and stained for Haematoxilin and Eosin. GM-CSF in serum were measured by ELISA. RNA was extracted from skin and the expression of GM-CSF and TGFa mRNA were examined by RT-PCR. Although the epidermis appeared normal, the dermis in exposed animals showed moderate thickening of the collagen bundles. Seven of 12 exposed male mice and 4 of 12 female mice showed significantly thickened collagen bundles. GM-CSF in serum were elevated in 10mg/L, but not significantly. RNA expressions for GM-CSF and TGFa were enhanced moderately in exposed groups compared to controls. These results indicate that a number of changes occur in the skin of As-exposed mice, including thickening of collagen bundles and increased growth factor expression. [Description provided by NIOSH]
• Subjects:
  Animals Arsenic Chemistry Chemistry, Analytic Environmental Monitoring Hazardous Substances Hypersensitivity Inorganic Chemicals Laboratories Occupational Health Poisoning Safety Skin Skin Diseases Toxicology

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• Keywords:
  Analytical-chemistry Analytical-methods Animal-studies Arsenic-compounds Arsenic-poisoning Chemical-analysis Dose-response Exposure-assessment Laboratory-animals Sensitivity-testing Sensitization Skin-disorders Skin-exposure Skin-irritants Skin-sensitivity Skin-tests Statistical-analysis Toxic-materials Toxins

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Louisiana ; North Carolina ; OSHA Region 3 ; OSHA Region 4 ; OSHA Region 6 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  48
• NIOSHTIC Number:
  nn:20028035
• Citation:
  Toxicologist 1999 Mar; 48(1-S):353
• CAS Registry Number:
  Arsenic (CAS RN 7440-38-2) ; Sodium arsenate (Na2HAsO4) (CAS RN 7778-43-0)
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 38th Annual Meeting, March 14-18, 1999, New Orleans, Louisiana
• Supplement:
  1-S
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ca6a1411c073c1aa91b544c062890a4728f746195f965445f2bc49f14ee7a3bfa1d1afb06eae80d34bad7d41e4dfc3f2fe0137fe52fbcb86272164a511991fe4
• Download URL:
  https://stacks.cdc.gov/view/cdc/189743/cdc_189743_DS1.pdf
• File Type:
  [PDF - 232.22 KB ]