The Human Mineral Dust-Induced Gene, Mdig, Is a Cell Growth Regulating Gene Associated With Lung Cancer

Details

• Personal Author:
  Castranova, Vincent ; Chen F ; Demers LM ; Lu Y ; Shi XL ; Stauffer JL ; Yuan BZ ; Zhang YD
• Description:
  Environmental or occupational exposure to mineral dusts, mainly silica and asbestos, is associated with an increased incidence of lung inflammation, fibrosis, and/or cancer. To better understand the molecular events associated with these pulmonary diseases, we attempted to identify genes that are regulated by mineral dusts. Using a differential display reverse transcription polymerase chain reaction technique and mRNAs of alveolar macrophages from both normal individuals and coal miners, we identified a novel mineral dust-induced gene named mdig, which had not been fully characterized. The expression of mdig mRNA was detected in alveolar macrophages from coal miners but not from normal subjects. The inducible expression of mdig could be observed in A549 cells exposed to silica particles in a time-dependent manner. The full-length mdig mRNA was expressed in human lung cancer tissues but was barely detectable in the adjacent normal tissues. In addition, a number of lung cancer cell lines constitutively express mdig. Alternative spliced transcripts of mdig were detected in some lung cancer cell lines. Silencing mdig mRNA expression in A549 lung cancer cells by siRNA-mediated RNA interference inhibits cell proliferation and sensitizes the cells to silica-induced cytotoxicity. These results suggest that the mdig gene may be involved in the regulation of cell growth and possibly the development of cancer. [Description provided by NIOSH]
• Subjects:
  Aluminum Silicates Asbestos Coal Coal Mining Dust Environmental Exposure Environmental Monitoring Genetics Lung Lung Diseases Minerals Mining Neoplasms Occupational Exposure Occupational Health Respiratory System Respiratory Tract Diseases Safety Silicon Dioxide

  More +
• Keywords:
  Asbestos-dust Cancer Cell-growth Coal-miners Dust-exposure Dusts Environmental-exposure Exposure-assessment Exposure-levels Genes Lung-cancer Lung-fibrosis Mineral-dusts Miners Occupational-exposure Pulmonary-system-disorders Respiratory-system-disorders Silica-dusts Silicates

  More +
• ISSN:
  0950-9232
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  24
• Issue:
  31
• NIOSHTIC Number:
  nn:20028019
• Citation:
  Oncogene 2005 Jul; 24(31):4873-4882
• Contact Point Address:
  F Chen, Pathology and Physiology Research Branch, National Institute for Occupational Safety and Health, Mail Stop 2015, 1095 Willowdale Road, Morgantown, WV 26505, USA
• Email:
  LFD3@cdc.gov
• CAS Registry Number:
  Asbestos (CAS RN 1332-21-4) ; Quartz (SiO2) (CAS RN 14808-60-7)
• Federal Fiscal Year:
  2005
• Peer Reviewed:
  True
• Source Full Name:
  Oncogene
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f85dc8fb462bbb772a39c11c0af2e6a091ef7c74bcf1fc3e69cd0e77dbc0f7740e801428ceb2fce016d24fe2120d71c8950c99490f9050f6805550c96fb71f91
• Download URL:
  https://stacks.cdc.gov/view/cdc/189729/cdc_189729_DS1.pdf
• File Type:
  [PDF - 1.99 MB ]