Ethanol-Mediated Induction of CYP1A1/2 in Rat Skeletal Muscle

Details

• Personal Author:
  Gannett PM ; Harsh V ; Miller MR ; Riggs JE ; Smith C ; Stamm SC ; Stauber W
• Description:
  This study tested the hypothesis that feeding rats an ethanol-containing diet would induce CYP2E1 and/or CYPIA 1/2 in skeletal muscle tissue; these cytochrome P450 enzymes were also analyzed in liver, as a positive control tissue. Enzymatic assays, p-nitrophenol (PNP, for CYP2E1) and ethoxyrdorufin O-deethylase (EROD, for CYPIA1/2), as well as Western blot analysis, demonstrated that a liquid diet containing 6% ethanol and delivered via a feeding system using glass bottles and rubber stoppers with a stainless steel tube (Feeding System 1) induced CYP2E1 and CYP1A1/2 in liver tissue. Immunocytochemistry studies confirmed induction of these liver enzymes by the ethanol-containing diet. Imrnunohistochemical analysis of skeletal musclee (soleus, plantaris, and diaphragm) indicated ethanol-containing diets administered by Feeding System 1 induced CYPIA1/2, and immunohistochemical staining was predominantly localized to capillaries currounding myofibers. However, antibodies to CYP2E1 did not detectably react with skeletal muscle tissue from animals receiving a control or ethanol-containing diet. Identical results were obtained when highly purified ethanol was preparedd and administered in the diets. However, when liquid diets were administered via Feeding System 2 (all-glads feeding system), CYP1A1/2 was not induced by ethanol-containing diets in liver or in skeletal muscle tissue; CYP2E1 was induced in liver by Feeding System 2. The results indicate that feeding ethanol can mediate induction of CYPIAl/2 in liver and in capillaries of skeletal muscle; however, this induction was not attributed to ethanol per se, but rather to an unidentified compound(s) apparently solubilized by 6% ethanol from robber stoppers or stainless steel tubing. [Description provided by NIOSH]
• Subjects:
  Animals Chemistry Chemistry, Analytic Environmental Monitoring Hazardous Substances Laboratories Musculoskeletal Diseases Musculoskeletal System Occupational Health Safety Toxicology

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• Keywords:
  Analytical-chemistry Analytical-methods Animal-studies Exposure-assessment Laboratory-animals Liver Skeletal-disorders Skeletal-system Skeletal-system-disorders Statistical-analysis Toxic-materials Toxins

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Louisiana ; OSHA Region 3 ; OSHA Region 6 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  98
• Volume:
  48
• NIOSHTIC Number:
  nn:20027989
• Citation:
  Toxicologist 1999 Mar; 48(1-S):98
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 38th Annual Meeting, March 14-18, 1999, New Orleans, Louisiana
• Supplement:
  1-S
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9b4e47fd1566282e7bf838f7cc86b5bd48bb660e44bc04af759d9f532e0478089e474db0383750e7e6169070d9a6ed94b6af9162393a0c5ea72cde238999fb73
• Download URL:
  https://stacks.cdc.gov/view/cdc/189717/cdc_189717_DS1.pdf
• File Type:
  [PDF - 184.87 KB ]