Mercaptobenzothiazole Allergenicity – Role of the Thiol Group

Details

• Personal Author:
  Chipinda I ; Siegel, Paul D. ; Simoyi RH ; Zhang XD
• Description:
  The rubber accelerator, 2-mercaptobenzothiazole (MBT), is known to cause allergic contact dermatitis (ACD), but the mechanism is unknown. The role of the thiol group in MBT's allergenicity was investigated in the present study. Guinea pigs were sensitized to MBT using a modified guinea pig maximization test (GPMT) and reactivity was assessed toward 2-mercaptobenzothiazole disulfide (MBTS), 2-hydroxybenzothiazole (HBT; thiol-substituted), 2-(methylthio)benzothiazole (MTBT; thiol-blocked), and benzothiazole (BT; thiol-lacking). MBT and MBTS, but not BT, HBT, or MTBT, elicited ACD in MBT-sensitized animals, demonstrating that the thiol group is critical to MBT's allergenicity. In addition, both MBT and MBTS were shown to inhibit both glutathione reductase and thioredoxin reductase, and thus contribute to the stability of MBT-protein mixed disulfides. It is concluded that the probable haptenation mechanism of MBT is through initial oxidation to MBTS with subsequent reduction to form mixed disulfides with proteins. [Description provided by NIOSH]
• Subjects:
  Dermatitis Hazardous Substances Irritants Occupational Health Risk Assessment Risk Factors Safety Skin Skin Diseases
• Keywords:
  Risk-analysis Risk-factors Skin-disorders Skin-exposure Skin-irritants Skin-sensitivity Toxic-effects
• ISSN:
  1556-9527
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Oregon ; OSHA Region 10 ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  103-116
• Volume:
  27
• Issue:
  2
• NIOSHTIC Number:
  nn:20034095
• Citation:
  Cutan Ocul Toxicol 2008 Jun; 27(2):103-116
• Contact Point Address:
  Paul D. Siegel, Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505-2888
• Email:
  pds3@cdc.gov
• CAS Registry Number:
  2-Mercaptobenzothiazole (CAS RN 149-30-4)
• Federal Fiscal Year:
  2008
• Peer Reviewed:
  True
• Source Full Name:
  Cutaneous and Ocular Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ab2aed0aa2fb9d6b818b6022b19ad1ccc0bd619558324bc3301b2867fbb36e7ec79710db5cf34b70b343903a36a27ce2edf7bc43c73c364df550e014eb380055
• Download URL:
  https://stacks.cdc.gov/view/cdc/189465/cdc_189465_DS1.pdf
• File Type:
  [PDF - 338.10 KB ]