The Effect of Cobalt, Tungsten, Tungsten-Cobalt, and Cobalt Chloride on L-Selectin Expression in K562, J-11, and Daudi Cells and on ICAM-1 Expression in L2 Cells

Details

• Personal Author:
  Rao KMK ; Starks KM ; Rao KMK ; Starks KM
• Description:
  We studied the effect of cobalt. tungsten and tungsten/cobalt particles on the expression of L-selectin at the protein level in a cell line (K562) transfected with a cDNA clone of L-selectin and in two lymphoid cell lines which express L-selectin constitutively (Daudi and J-11). Cell surface receptor expression was determined by flow cytometry. The cell lines were incubated with 5IJg/mi of the metal par1\cles for 24, 48 and 72 hours. There was no difference in L-selectin expression in control and experimental samples. We also studied the effect of these metals on the expression of ICAM-1 in a rat lung epithelial-like cell line (L-2, ATCC) at the mRNA level. mRNA levels were measured by semi- quantitative RT -PCR. ICAM-1 mRNA was analyzed in L2 ceils after 24h Incubation with metal particles at 1 ug/ml, 3 ug/mi. 5 ug/mi. and 7 ug/ml No significant differences were found in the ICAM-1 mRNA levels. In contrast, CoCl2 at a concentration of 50 uM caused a significant decrease in ICAM-1 mRNA levels (40% of the control value; P < 0.004, n ; 4). These data indicate that in short-term exposure experiments the hard metal particles have no significant effect on the expression of selectin and ICAM-1 family of adhesion molecules. The pathophysiology of hard metal disease remains to be elucidated. [Description provided by NIOSH]
• Subjects:
  Dust Metals Metals, Heavy Occupational Health Particulate Matter Safety
• Keywords:
  Cell-function Cellular-function Cobalt-compounds Dust-particles Genotoxic-effects Heavy-metals Metal-compounds Metal-dusts Metallic-compounds Metallic-dusts Particulate-dust Respirable-dust Tungsten-compounds

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• ISSN:
  1059-1524
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings ; Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  8
• NIOSHTIC Number:
  nn:20034047
• Citation:
  Mol Biol Cell 1997 Nov; 8(Suppl):1689
• CAS Registry Number:
  Cobalt (CAS RN 7440-48-4) ; Cobalt chloride (CoCl2) (CAS RN 7646-79-9) ; Tungsten (CAS RN 7440-33-7) ; Cobalt (CAS RN 7440-48-4) ; Cobalt chloride (CoCl2) (CAS RN 7646-79-9) ; Tungsten (CAS RN 7440-33-7)
• Federal Fiscal Year:
  1998
• Peer Reviewed:
  False
• Source Full Name:
  Molecular Biology of the Cell
• Supplement:
  Suppl
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:da2f0dee7ae8504ce6c6a74507d5cf757de847435462ed7a483c0c75cd8f4f3a67890278a7de572a220671958e15f99dac99f7aa837a3411c48dbd2b38f76548
• Download URL:
  https://stacks.cdc.gov/view/cdc/189425/cdc_189425_DS1.pdf
• File Type:
  [PDF - 199.57 KB ]