Dietary Restriction Mitigates Ozone-Induced Lung Inflammation in Rats: A Role for Endogenous Antioxidants

Details

• Personal Author:
  Crissman K ; Hatch G ; Kari F ; Luster M ; Simeonova PP ; Slade R
• Description:
  Studies were undertaken to determine whether dietary restriction protects against acute pulmonary oxidant challenge, Male F344 rats were ed NIH-31 diet either ad libitum or at restricted levels equal to 75% that of ad libitum intake. After 3 wk of dietary adaptation, animals were exposed by inhalation to 2.0 ppm ozone (O-3) for 2 h or chamber air and evaluated for cellular and biochemical indices of pulmonary toxicity. Compared to air controls, bronchoalveolar lavage fluid (BALF) from O-3 exposed ad libitum fed rats contained increased protein (145 versus 380 mu g/ml), PMN infiltration (0 versus 11%) and fibronectin (45 versus 607 U/ml). Diet restriction abrogated these indicators of pulmonary inflammation induced by ozone. Binding of O-18(3) to BALF protein and cells was significantly decreased in diet restricted rats while BALF ascorbate and glutathione levels, bill not alpha-tocopherol or urate, were elevated compared to an libitum fed rats. Taken together, these results indicate that dietary restriction affords protection against O-3-induced oxidant toxicity. Protection is mediated partially by increases in ascorbate in the fluid bathing the lung surface, thereby providing an antioxidant sink which minimizes the ability of O-3 to reach biological targets. [Description provided by NIOSH]
• Subjects:
  Air Pollution Air Pollution, Indoor Biochemistry Chemistry Environmental Monitoring Hazardous Substances Hypersensitivity Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Air-quality Air-sampling Biochemical-analysis Breathing Chemical-hypersensitivity Chemical-properties Inhalation-studies Laboratory-animals Laboratory-testing Pulmonary-disorders Pulmonary-function Pulmonary-system-disorders Toxic-effects Toxic-vapors Toxins

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• ISSN:
  1044-1549
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  17
• Issue:
  6
• NIOSHTIC Number:
  nn:20033971
• Citation:
  Am J Respir Cell Mol Biol 1997 Dec; 17(6):740-747
• Contact Point Address:
  M Luster, NIOSH, Health Effects Research Laboratory, Toxicol & Molecular Biology Branch, 1095 Willowdale Rd, Mailstop 3014, Morgantown, WV 26505
• Email:
  myl6@cdc.gov
• Federal Fiscal Year:
  1998
• Peer Reviewed:
  True
• Source Full Name:
  American Journal of Respiratory Cell and Molecular Biology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:51d7b297330bf94b203b635e619949454058caa71baa41bb3a02d076886899de673cd634ef828d61d29be9e6af5d8fd59fbe235ec181f220e31c618f10fdbb32
• Download URL:
  https://stacks.cdc.gov/view/cdc/189366/cdc_189366_DS1.pdf
• File Type:
  [PDF - 98.96 KB ]