Effects of Ozone Inhalation on Reactivity to Methacholine, Epithelium-Derived Relaxing Factor (EpDRF) Release, and Neurogenic Responses in Guinea-Pig Tracheal Preparations In Vitro

Details

• Personal Author:
  Fedan, Jeffery S. ; Frazer DG ; Hubbs A ; Millecchia LL ; Orsini LE ; Yuan LX
• Description:
  We examined the effects of ozone (O3) inhalation on reactivity to methacholine (MCh) and release of EpDRF in guinea-pig airways using the isolated, perfused trachea (IPT), and the effects of O3 on electric field-stimulated (EFS) neurogenic responses of tracheal strips (TS). Animals were exposed to 3 ppm O3 or air for I hr; the trachea was removed immediately (0 hr), 2 hr, or 18 hr after exposure. Concentration-response curves for serosal (extraluminal, EL) and mucosal (intraluminal, IL.) addition of MCh, and EpDRF-induced relaxation induced by adding IL NaCl to MCh-contracted tracheas; were obtained from IPT. Frequency-related biphasic contractile/relaxant responses to EFS were obtained from resting and MCh-contracted TS. At 0 and 2 hr post-exposure, the sensitivity of IPT to IL-applied MCh was increased in the presence but not the absence of the epithelium; reactivity to EL MCh was not affected. EpDRF-induced relaxation responses were attenuated by O3-treatment. By 18 hr, reactivity to IL MCh and EpDRF-induced relaxation responses were not different from air-breathing control levels. O3-treatment had no effect on EFS-induced contractile or relaxant responses of TS: These results suggest that acute exposure to O3 induces a transient increase in mucosal permeability to MCh and. a decrease in EpDRF release. Both changes could contribute to airway hyperreactivity observed after O3-exposure. However, excitatory and inhibitory nerves are not are not affected by O3-exposure. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Air Pollution, Indoor Hypersensitivity Laboratories Lung Lung Diseases Nervous System Nervous System Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Air-contamination Breathing Chemical-hypersensitivity In-vitro-study Inhalation-studies Laboratory-animals Laboratory-testing Lung-disease Lung-irritants Nervous-system-disorders Neurological-system Pulmonary-system-disorders

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• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  10
• Issue:
  3
• NIOSHTIC Number:
  nn:20034880
• Citation:
  FASEB J 1996 Mar; 10(3):A168
• CAS Registry Number:
  Methacrolein (CAS RN 78-85-3) ; Ozone (CAS RN 10028-15-6)
• Federal Fiscal Year:
  1996
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e991a656bdd31606419cfdb03bae2d9c4b3d8e344192a7405f50b4faaeef01af8c4e7db5c8a5b3dec77b72e4e88e035ddd1f8d003d0d80707dd64e41cc3e4015
• Download URL:
  https://stacks.cdc.gov/view/cdc/186925/cdc_186925_DS1.pdf
• File Type:
  [PDF - 480.42 KB ]