The Utility of Epithelial-Cell Micronuclei in the Assessment of Intermittent Exposures

Details

• Personal Author:
  Boeniger, Mark F. ; Levine AJ ; Salvan A ; Schulte, Paul A. ; Suruda, Anthony J. ; Talaska G
• Description:
  Epithelial-cell micronuclei (MN) are potentially useful markers of occupational exposure to genotoxicants. With intermittent exposures, cells sampled either before or after a specific time interval, reflecting the time it takes for damaged cells to become available at the epithelial surface, are unlikely to be exposure-related. It may then be important to conduct an exposure-window analysis, with the goal of identifying the relevant exposures.We re-analysed individual exposure data from a previous study (Suruda et al. 1993) of MN formation in 22 male mortuary science students exposed to formaldehyde during a 90-day embalming class. We conducted an exposurewindow analysis and compared the results with those obtained with 90-day cumulative exposure. The window widths varied between 7 and 25 days, in 1 day increments, assuming a constant 7-day cell-cycle. We assessed the fit (likelihood-ratio test) of a linear regression model, regressing the change in buccal MN prevalence on formaldehyde exposure, using both asymptotic and non-asymptotic methods. Exposures defined from 7-15 to 7-18 days before specimen collection provided a slightly better fit than the 90-day cumulative exposure, with a doubling of the regression coefficient for the exposure effect (for the 7-16-days window LR = 5.32, p = 0.032, coefficient = 0.088 MN per 1000 cells per ppm-hr; 95% CI = 0.014, 0.16; for the 90-day cumulative exposure LR = 4.44, p = 0.048, coefficient = 0.045 MN per 1000 cells per ppm-hr, 95% CI = 0.0038, 0.086). Although hampered by the small number of subjects, these results reinforce the potential importance of exposure timing. [Description provided by NIOSH]
• Subjects:
  Environmental Monitoring Formaldehyde Occupational Exposure Occupational Health Risk Assessment Safety Skin Workplace
• Keywords:
  Cell-damage Exposure-assessment Formaldehydes Gene-mutation Genotoxic-effects Occupational-exposure Occupational-health Risk-analysis Skin-exposure Skin-irritants Workplace-studies

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• ISSN:
  1354-750X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DSHEFS - Division of Surveillance, Hazard Evaluations, and Field Studies ; EID - Education and Information Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  135-138
• Volume:
  2
• Issue:
  2
• NIOSHTIC Number:
  nn:20033969
• Citation:
  Biomarkers 1997 Mar; 2(2):135-138
• CAS Registry Number:
  Formaldehyde (CAS RN 50-00-0)
• Federal Fiscal Year:
  1997
• Peer Reviewed:
  True
• Source Full Name:
  Biomarkers
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:72fdc520ecd4ce536fed96a5d87785df06b96ef5cd59d4ec5f04b57fb7c52ed47b7b47093dddcb1f9666114f7ad076624b23462a7cab143b049b28a0518d58ca
• Download URL:
  https://stacks.cdc.gov/view/cdc/189365/cdc_189365_DS1.pdf
• File Type:
  [PDF - 311.21 KB ]