Distribution of p53 and K-Ras Mutations in Human Lung Cancer Tissues

Details

• Personal Author:
  Chen JK ; Gao HG ; Ong T ; Rayappa C ; Song B ; Stewart J ; Whong, Wen-Zong
• Description:
  Studies were performed to examine the mutational pattern of K-ras exons 1 and 2 and p53 exons 5-8 in lung cancer tissues from 27 Chinese patients (10 smokers, 17 non-smokers) using single-stranded conformational polymorphism and DNA sequencing. K-ras mutations were found in 13/27 tumors (48%); all mutations were clustered in exon 1 and distributed between codons 9 and 32. The frequency and number of patients with K-ras mutations between smokers and non-smokers were not different, except that a high frequency of G --> A transitions (11/11) was found in non-smokers. Among cell types, K-ras mutations were found in 7/13 (54%) squamous cell carcinoma (SC) and 5/12 (42%) adenocarcinoma (AC) patients. A --> T transversions (all six transversions) were present only in SC. In p53, 18/27 (67%) tumors contained mutations in exons 7 and 8, frequently at codons 226, 270, 275 and 281. The number of tumors with p53 mutations in smokers (70%) and in non-smokers (65%) was similar, and the mutation frequency did not differ except for a higher number of G --> A (6/7) and T --> C (5/6) transitions in non-smokers. Among cell types, the number of tumors with p53 mutations was 9/13 (69%) in SC and 8/12 (67%) in AC. The A --> G (11/16) transitions and A --> C (4/4) transversions in p53 were more frequent in SC than in AC (P < 0.04 for A --> G; P < 0.02 for A --> C). The varying mutation patterns in both the K-ras and p53 genes between smokers and non-smokers and among cell types suggest that other than cigarette smoke, environmental and dietary factors may also be involved in the genesis of lung cancer among these patients. Description provided by NIOSH
• Subjects:
  • Carcinogens
  • Lung
  • Lung Diseases
  • Mortality
  • Neoplasms
  • Occupational Health
  • Respiratory System
  • Respiratory Tract Diseases
  • Safety
• Keywords:
  • Cell-damage
  • Cell-function
  • Lung-cancer
  • Lung-disorders
  • Respiratory-system-disorders
  • Pulmonary-system-disorders
  • Cancer-rates
  • Mortality-rates
  • Gene-mutation
  • Carcinogens
• ISSN:
  0143-3334
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  473-478
• Volume:
  18
• Issue:
  3
• NIOSHTIC Number:
  nn:20033945
• Citation:
  Carcinogenesis 1997 Mar; 18(3):473-478
• Contact Point Address:
  T. Ong, Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WV 26505-2845, USA
• Federal Fiscal Year:
  1997
• Peer Reviewed:
  True
• Source Full Name:
  Carcinogenesis
• Download URL:
  https://stacks.cdc.gov/view/cdc/189343/cdc_189343_DS1.pdf
• File Type:
  [PDF - 138.90 KB]
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c02d48f180171993d44b542a283b0198447ed0c22edb3ed26afdba85fb4b49ccd542033b3a0ca6602ed395c2bfa5f77b44281978bd3cf08560e5fe739ca98d9a