Distribution of p53 and K-Ras Mutations in Human Lung Cancer Tissues

Details

• Personal Author:
  Chen JK ; Gao HG ; Ong T ; Rayappa C ; Song B ; Stewart J ; Whong, Wen-Zong
• Description:
  Studies were performed to examine the mutational pattern of K-ras exons 1 and 2 and p53 exons 5-8 in lung cancer tissues from 27 Chinese patients (10 smokers, 17 non-smokers) using single-stranded conformational polymorphism and DNA sequencing. K-ras mutations were found in 13/27 tumors (48%); all mutations were clustered in exon 1 and distributed between codons 9 and 32. The frequency and number of patients with K-ras mutations between smokers and non-smokers were not different, except that a high frequency of G --> A transitions (11/11) was found in non-smokers. Among cell types, K-ras mutations were found in 7/13 (54%) squamous cell carcinoma (SC) and 5/12 (42%) adenocarcinoma (AC) patients. A --> T transversions (all six transversions) were present only in SC. In p53, 18/27 (67%) tumors contained mutations in exons 7 and 8, frequently at codons 226, 270, 275 and 281. The number of tumors with p53 mutations in smokers (70%) and in non-smokers (65%) was similar, and the mutation frequency did not differ except for a higher number of G --> A (6/7) and T --> C (5/6) transitions in non-smokers. Among cell types, the number of tumors with p53 mutations was 9/13 (69%) in SC and 8/12 (67%) in AC. The A --> G (11/16) transitions and A --> C (4/4) transversions in p53 were more frequent in SC than in AC (P < 0.04 for A --> G; P < 0.02 for A --> C). The varying mutation patterns in both the K-ras and p53 genes between smokers and non-smokers and among cell types suggest that other than cigarette smoke, environmental and dietary factors may also be involved in the genesis of lung cancer among these patients. [Description provided by NIOSH]
• Subjects:
  Carcinogens Lung Lung Diseases Mortality Neoplasms Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Cancer-rates Cell-damage Cell-function Gene-mutation Lung-cancer Lung-disorders Mortality-rates Pulmonary-system-disorders Respiratory-system-disorders
• ISSN:
  0143-3334
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  473-478
• Volume:
  18
• Issue:
  3
• NIOSHTIC Number:
  nn:20033945
• Citation:
  Carcinogenesis 1997 Mar; 18(3):473-478
• Contact Point Address:
  T. Ong, Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, Morgantown, WV 26505-2845, USA
• Federal Fiscal Year:
  1997
• Peer Reviewed:
  True
• Source Full Name:
  Carcinogenesis
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c02d48f180171993d44b542a283b0198447ed0c22edb3ed26afdba85fb4b49ccd542033b3a0ca6602ed395c2bfa5f77b44281978bd3cf08560e5fe739ca98d9a
• Download URL:
  https://stacks.cdc.gov/view/cdc/189343/cdc_189343_DS1.pdf
• File Type:
  [PDF - 138.90 KB ]