Detection of Genomic Instability in Lung Cancer Tissues by Random Amplified Polymorphic DNA Analysis

Details

• Personal Author:
  Ong T ; Qian H ; Song B ; Whong W ; Wu ZL
• Description:
  Genomic instability resulting in multiple mutations is believed to be a driving force in the carcinogenic process. In this study, the random amplified polymorphic DNA (RAPD) technique, a simple PCR-based DNA polymorphism assay system, was used for detecting genomic instability in lung cancer tissues. DNAs from 20 lung cancer (18 non-small cell lung cancers and two small cell lung cancers) and their corresponding normal tissues were amplified individually by RAPD with seven different 10-base arbitrary primers. PCR products from RAPD were electrophoretically separated in agarose gels and banding profiles were visualized by ethidium bromide staining. The ability to detect genomic instability in 20 cancer tissues by each single primer ranged from 15 to 75%. DNA changes were detected by at least one primer in 19 (95%) cancer tissues. These results seem to indicate that genomic rearrangement is associated with lung carcinogenesis and that RAPD analysis is useful for the detection of genomic instability in lung cancer tissues. [Description provided by NIOSH]
• Subjects:
  Carcinogens Genetics Lung Lung Diseases Neoplasms Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Carcinogenesis Carcinogenicity Genes Lung-cancer Lung-cells Lung-disease Lung-disorders Pulmonary-system-disorders Respiratory-system-disorders
• ISSN:
  0143-3334
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  233-235
• Volume:
  19
• Issue:
  1
• NIOSHTIC Number:
  nn:20024942
• Citation:
  Carcinogenesis 1998 Jan; 19(1):233-235
• Contact Point Address:
  Toxicology and Molecular Biology Branch, Health Effects Laboratory Division. National Institute for Occupational Safety and Health. Morgantown WV 26505, USA
• Email:
  too2@cdc.gov
• Federal Fiscal Year:
  1998
• Peer Reviewed:
  True
• Source Full Name:
  Carcinogenesis
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c519a97c81891f48e1048c103e6a48a03169e7b5923872e56ed6819815bdd13cc34f2317bdc4ec968b93c1982f34cbb07d7ec98c69489a305ca5c404b1f8e20a
• Download URL:
  https://stacks.cdc.gov/view/cdc/196503/cdc_196503_DS1.pdf
• File Type:
  [PDF - 113.03 KB ]