Non-Random Chromosomal Changes in High and Low Invasive Tumor Cells Derived from Early Passage Mouse Lung Adenocarcinoma Cell Cultures

Details

• Personal Author:
  Ensell MX ; Jefferson AM ; Kashon ML ; Lowry DT ; Reynolds SH ; Sargent LM ; Senft JR ; Tyson FL
• Description:
  In an attempt to identify chromosomal changes that may be involved with invasive and/or metastatic properties, we used Spectral Karyotyping to analyze nine pairs of high and low invasive tumor cell cultures derived from early passage (3 to 5 passages from the primary tumor) mouse lung adenocarcinoma cell lines. The high and low invasive cultures were obtained by a Gel Matrix technique. The cells with high invasive property penetrate the gel matrix while the cells with low invasive property remain on the surface. Seven of the nine original lung adenocarcinomas were from the A/J mouse, a strain highly susceptible to lung carcinoma. Two of the nine original lung adenocarcinomas were from B6C3F1 mice, an F1 hybrid resulting from a cross between two resistant strains, C57BU6J and C3H/Hen. At least twenty metaphase spreads were observed for each culture: A chromosomal event that occurred in over 40% of the cells from a specific culture was scored as a positive event. Regression Tree analysis showed that duplication of chromosome 1 is significant in high invasive cultures (9/9) compared to low invasive cultures (3/9). Mapping with fluorescent labeled genomic probes identified the minimal region of duplication to be 73 to 94 centimorgan on chromosome 1, which includes a candidate Cyclooxyenase 2 (C0X2) gene. Further study for the expression of C0X2 is under investigation. Duplication of chromosome 15 was also found to be significantly higher in high invasive (8/9) than in low invasive (4/9) cultures. Our results indicate that chromosome 1 and 15 may be involved with the invasiveness and the candidate genes on these chromosome regions may play role for invasive and/or metastatic properties. [Description provided by NIOSH]
• Subjects:
  Animals Chromosomes DNA Genetics Laboratories Lung Lung Diseases Neoplasms Occupational Health Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Animal-studies Cancer Cancer-rates Chromosome-damage Chromosome-disorders DNA-damage Genetic-factors In-vitro-study Laboratory-animals Lung-cancer Models Pulmonary-system-disorders Respiratory-system-disorders

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• ISSN:
  0197-016X
• Publisher:
  American Association for Cancer Research
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  California ; OSHA Region 3 ; OSHA Region 9 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  43
• NIOSHTIC Number:
  nn:20033744
• Citation:
  Proceedings of the 93rd American Association for Cancer Research Annual Meeting, April 6-10, 2002, San Francisco, California. Philadelphia, PA: American Association for Cancer Research, 2002 Apr; 43:634
• CAS Registry Number:
  4,4'-Diaminodiphenyl (CAS RN 92-87-5)
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  False
• Source Full Name:
  Proceedings of the 93rd American Association for Cancer Research Annual Meeting, April 6-10, 2002, San Francisco, California
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ca600120776d4a74704b33f1bb920996c4aa810b39258b2073a84844ab817188a7d26bff113dd01728af0b76bd952807a3ef6aba4a7153586526f0aa7767dc4d
• Download URL:
  https://stacks.cdc.gov/view/cdc/189201/cdc_189201_DS1.pdf
• File Type:
  [PDF - 373.37 KB ]