Use of a Human Microsomal Library for Metabolite Elucidation, Assay Development and Biological Monitoring of Occupationally Relevant Compounds

Details

• Personal Author:
  Biagini, Raymond E. ; Snawder, John E. ; Striley CAF
• Description:
  Immunochemical biomonitoring is an effective tool for assessing exposure to agrochemicals. Effective biomonitoring requires knowledge of agent-specific human pharmacokinetics We have procured a library of human tissue subfractions (hepatic, renal, pulmonary and jejunal) to serve as an invitro surrogate of human metabolism. Characterization of these tissues for Phase I and Phase II metabolic enzymes has been performed to elucidate variation in metabolism. The impact of individual variation on the metabolism of occupational/environmental chemicals can be estimated by accurately quantifying the expression of the pertinent enzyme(s) in human tissues. These data can be combined with data describing the specific activity of CYP forms towards substrates to develop metabolic rates, and their ranges, useful for the development of suitable biomarkers to estimate exposure to xenobiotics. Microsomal assay and HPLC analysis was used to determine human metabolism of azinphosmethyl, piperalin and bromopropane. Candidate biomarkers were then used to develop ELISAs for urinary analysis [Description provided by NIOSH]
• Subjects:
  Agriculture Biomarkers Chemistry Chemistry, Analytic Environmental Monitoring Occupational Health Safety Urine
• Keywords:
  Agricultural-chemicals Analytical-chemistry Analytical-processes Biological-monitoring Cell-metabolism Environmental-health-monitoring Humans Urinalysis Urine-chemistry
• ISSN:
  0065-7727
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  221
• NIOSHTIC Number:
  nn:20033650
• Citation:
  Abstr Pap - Am Chem Soc 2001 Apr; 221(Pt 2):U59
• Contact Point Address:
  Cynthia AF Striley, Biomonitoring and Health Assessment Branch, National Institute for Occupational Safely and Health, 4676 Columbia Parkway, Mailstop C-26, Cincinnati, OH 45226
• Email:
  chs3@cdc.gov
• Federal Fiscal Year:
  2001
• Peer Reviewed:
  False
• Part Number:
  2
• Source Full Name:
  Abstracts of Papers - American Chemical Society
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:95aa166371f7c87b872eb752a4cbdd1b8b6595a360a344df2f8c25ee2a6fbccc11d6d026af61e640332b346aa5d627e4820bcd8c8620a06f6146973bad1123cd
• Download URL:
  https://stacks.cdc.gov/view/cdc/189135/cdc_189135_DS1.pdf
• File Type:
  [PDF - 131.72 KB ]