Inhalation of Carbon Nanotubes Induces Oxidative Stress and Cytokine Response Causing Respiratory Impairment and Pulmonary Fibrosis in Mice

Details

• Personal Author:
  Arepalli S ; Baron, Paul A. ; Castranova, Vincent ; Chen T ; Deye G ; Frazer D ; Gorelik O ; Hubbs, Ann F. ; Johnson V ; Kadiiska M ; Kagan VE ; Kisin E ; Mason R ; Maynard A ; Mercer RR ; Mouithys-Mickalad A ; Murray AR ; Shvedova AA ; Stadler K ; Stone S
• Description:
  Due to their unique physico-chemical, electrical, mechanical and thermal properties, single walled carbon nanotubes (SWCNT) have numerous applications in electronics, aerospace devices, and computers, chemical, polymer and pharmaceutical industries. Previously, we have reported that pharyngeal exposure of C57BL/6 mice to SWCNT caused dose-dependent formation of granulomatous bronchial interstitial pneumonia, fibrosis, oxidative stress, acute inflammatory/cytokine responses and decrease in pulmonary function. In this study, we investigated whether whole-body inhalation exposure to aerosolized SWCNT (using generation system developed at NIOSH, Baron et al., 2007) caused pulmonary toxicity and early onset of fibrogenic response similar to adverse pulmonary toxicity reported previously. Here, we report that both inhalation and aspiration exposures to unrefined SWCNT (HiPco, CNI, Inc, TX) resulted in an augmentation of biomarkers of inflammation and oxidative stress as evidenced by ESR detection of POBN-spin trapped carbon centered radicals and significant depletion of antioxidants in mouse lungs, recruitment of inflammatory cells and increase in pro-inflammatory cytokines assessed in the bronchoalveolar lavage (BAL) fluid, and early onset of granulomatous lesions and interstitial pulmonary fibrosis. The chain of pathological events in both exposure routes was realized through synergized interactions of early inflammatory response and oxidative stress culminating in the development of multifocal granulomatous pneumonia and robust interstitial fibrosis. [Description provided by NIOSH]
• Subjects:
  Aerosols Biological Factors Environmental Monitoring Irritants Laboratories Lung Lung Diseases Occupational Health Particulate Matter Respiratory Hypersensitivity Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Aerosol-particles Aerosol-sampling Air-quality-measurement Biological-effects Biological-factors Biological-monitoring Breathing Cell-biology Fibrogenicity Inhalation-studies Laboratory-animals Nanotechnology Particulate-dust Particulates Pulmonary-disorders Pulmonary-system Pulmonary-system-disorders Respiratory-hypersensitivity Respiratory-irritants Respiratory-system-disorders

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  Ohio ; OSHA Region 10 ; OSHA Region 3 ; OSHA Region 5 ; Pennsylvania ; Washington ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  102
• Issue:
  1
• NIOSHTIC Number:
  nn:20033629
• Citation:
  Toxicologist 2008 Mar; 102(1):307
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 47th Annual Meeting and ToxExpo, March 16-20, 2008, Seattle, Washington
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:97ea96b2c13e95c77738e467e4fd1d286ebbf351d712b58050cc24b212ad1e172264424a526e87dab8d80921a8aaaf60279f0d8220e0ad390a87966e9c7357fb
• Download URL:
  https://stacks.cdc.gov/view/cdc/189115/cdc_189115_DS1.pdf
• File Type:
  [PDF - 7.72 MB ]