Biomarkers of Oxidative Stress and DNA Damage in Agricultural Workers: A Pilot Study

Details

• Personal Author:
  Kisby GE ; Koshy M ; Kow YW ; Lasarev M ; McCauley L ; Muniz JF ; Nazar-Stewart V ; Scherer J
• Description:
  Oxidative stress and DNA damage have been proposed as mechanisms linking pesticide exposure to health effects such as cancer and neurological diseases. A study of pesticide applicators and farmworkers was conducted to examine the relationship between organophosphate pesticide exposure and biomarkers of oxidative stress and DNA damage. Urine samples were analyzed for OP metabolites and 8-hydroxy-2'-deoxyguanosine (8-OH-dG). Lymphocytes were analyzed for oxidative DNA repair activity and DNA damage (Comet assay), and serum was analyzed for lipid peroxides (i.e., malondialdehyde, MDA). Cellular damage in agricultural workers was validated using lymphocyte cell cultures. Urinary OP metabolites were significantly higher in farmworkers and applicators (p<0.001) when compared to controls. 8-OH-dG levels were 8.5 times and 2.3 times higher in farmworkers or applicators (respectively) than in controls. Serum MDA levels were 4.9 times and 24 times higher in farmworkers or applicators (respectively) than in controls. DNA damage (Comet assay) and oxidative DNA repair were significantly greater in lymphocytes from applicators and farmworkers when compared with controls. Markers of oxidative stress (i.e., increased reactive oxygen species and reduced glutathione levels) and DNA damage were also observed in lymphocyte cell cultures treated with an OP. The findings from these in vivo and in vitro studies indicate that organophosphate pesticides induce oxidative stress and DNA damage in agricultural workers. These biomarkers may be useful for increasing our understanding of the link between pesticides and a number of health effects. [Description provided by NIOSH]
• Subjects:
  Agriculture Environmental Monitoring Epidemiology Insecticides Occupational Exposure Occupational Health Safety
• Keywords:
  Agricultural-workers Biological-monitoring DNA Damage Exposure-assessment Occupational-exposure Pesticides-and-agricultural-chemicals
• ISSN:
  0041-008X
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 10 ; Washington
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  97-107
• Volume:
  227
• Issue:
  1
• NIOSHTIC Number:
  nn:20033612
• Citation:
  Toxicol Appl Pharmacol 2008 Feb; 227(1):97-107
• Contact Point Address:
  Center for Research on Occupational and Environmental Toxicology (CROET), Oregon Health & Science University, Portland, OR 97239
• Email:
  munizj@ohsu.edu
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Washington
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Toxicology and Applied Pharmacology
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1e477a2807eae7cc8800190ac7dc5ffac38d3043ad89e59ed392596639fecfe42f977289d16efd055434000d356daede6d8b8caacce5d449ef2a81de8dac5d4e
• Download URL:
  https://stacks.cdc.gov/view/cdc/189109/cdc_189109_DS1.pdf
• File Type:
  [PDF - 562.24 KB ]