Single-Walled Carbon Nanotubes: Geno- and Cyto-Toxic Effects in Lung Fibroblast V79 Cells

Details

• Personal Author:
  Arepalli S ; Castranova, Vincent ; Gorelik O ; Kagan V ; Keane M ; Kisin E ; Murray A ; Schwegler-Berry D ; Shi X ; Shvedova A ; Wallace W
• Description:
  With the development of nanotechnology, there is a tremendous growth of the application of nanomaterials, which increases the risk of human exposure to these nanomaterials through inhalation, ingestion and dermal penetration. Among different types of nanoparticles, single walled carbon nanotubes (SWCNT) with extremely small size (1 nm in diameter) exhibit extraordinary properties and offer possibilities to create materials with astounding features. Since the release of nanoparticles in an enclosed environment is of great concern, a study of possible genotoxic effects is important. Our previous data showed that pharyngeal aspiration of SWCNT elicited pulmonary effects in C57BL/6 mice that were characterized by a robust, acute inflammatory reaction with an early onset and resulting in progressive interstitial fibrogenic response, and the formation of granulomas. In the present study, the genotoxic potential of SWCNT was evaluated in vitro. The genotoxic effects of nanoparticles were examined using three different test systems: the comet assay and micronucleus (MN) test in a lung fibroblast (V79) cell line, and the Salmonella gene mutation assay in strains YG1024/YG1029. Cytotoxicity tests showed loss of viability in a concentration- and time-dependent manner after exposure of cells to SWCNT. Results from the comet assay demonstrated the induction of DNA damage after only 3 hr incubation with 96 ug/cm2 of SWCNT. The MN test indicated some but not significant micronucleus induction by SWCNT in the V79 cell line at the highest concentrations tested. With two different strains of Salmonella typhimurium, no mutations were found following SWCNT exposure. [Description provided by NIOSH]
• Subjects:
  Dust Laboratories Lung Lung Diseases Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Cell-alteration Cell-biology Cell-transformation Cellular-reactions Fibrogenicity Fibrous-dusts Genotoxic-effects Genotoxicity Laboratory-animals Lung-cells Lung-disease Lung-fibrosis Lung-irritants Molecular-biology Molecular-structure Nanotechnology Particle-aerodynamics Particulate-dust Particulates Pathogenicity Pathogens Pathomorphology Pulmonary-system-disorders

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 10 ; OSHA Region 3 ; Pennsylvania ; Washington ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  90
• Volume:
  102
• Issue:
  1
• NIOSHTIC Number:
  nn:20033548
• Citation:
  Toxicologist 2008 Mar; 102(1):90
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 47th Annual Meeting and ToxExpo, March 16-20, 2008, Seattle, Washington
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:802a5193e9c0dfe793471e1edcff43887ad6233e0cdab0dc5c8dcf17e5a33fe25630fc182a3d5a77d4390515281604d9f4deb112beaadad1f1364a9221b1af85
• Download URL:
  https://stacks.cdc.gov/view/cdc/189082/cdc_189082_DS1.pdf
• File Type:
  [PDF - 7.81 MB ]