Surfactant Protein-D as a Possible Serum Biomarker for Amiodarone-Induced Pulmonary Toxicity in a Rat Model

Details

• Personal Author:
  Mason RJ ; Miles PR ; Reasor MJ ; Shannon K ; Taylor MD
• Description:
  Amiodarone (AD), an important antiarrhythmic drug, can induce pulmonary toxicity characterized by alveolar and interstitial inflammation and subsequent fibrosis. To date, no biomarkers have been identified to allow monitoring of the onset and/or progression of amiodarone-induced pulmonary toxicity (AIPT). Animal models of AIPT have been developed to study the disorder in the laboratory. In the present study, we utilized a rat model of AIPT where AD was instilled intratracheally (i.t.) (6.25 mg/kg in sterile water) on days 0 and 2. Controls received the water vehicle. On day 28, the level of right lung hydroxyproline, a biochemical marker for fibrosis was significantly elevated compared to controls indicating that pulmonary fibrosis had developed. Analysis of bronchoalveolar lavage fluid revealed a variable neutrophilic and strong eosinophilic inflammation through day 7. Surfactant protein-D (SP-D) was significantly elevated 2 to 3-fold in serum from AD-treated compared to control rats on days 5, 6 & 7 of the study. Dosing protocols where fibrosis did not develop (i.e., a single i.t. instillation of AD or when AD was given orally) did not result in an elevation of serum SP-D. The results of this study indicate that SP-D may serve as a serum biomarker to monitor the onset of AIPT. [Description provided by NIOSH]
• Subjects:
  Biological Factors Biomarkers Epidemiology Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Risk Assessment Risk Factors Safety

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• Keywords:
  Biological-factors Pulmonary-disorders Pulmonary-function Pulmonary-system Pulmonary-system-disorders Respiratory-system-disorders Risk-analysis Risk-factors Statistical-analysis
• ISSN:
  1073-449X
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  159
• Issue:
  3
• NIOSHTIC Number:
  nn:20033446
• Citation:
  Am J Respir Crit Care Med 1999 Jan; 159(3)(Suppl):A393
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  False
• Source Full Name:
  American Journal of Respiratory and Critical Care Medicine
• Supplement:
  Suppl
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f5970f929557613b2efbe073b5665d5fd904adb592b2e060ed01823e3d930b47cdd4af8bec56836ce1013daddbadba93d76e0ca84be75cb082d2fb5d5c6f48d7
• Download URL:
  https://stacks.cdc.gov/view/cdc/189025/cdc_189025_DS1.pdf
• File Type:
  [PDF - 871.70 KB ]