Analysis of Immunotoxicity by Enumeration of Antibody-Producing B Cells

Details

• Personal Author:
  Anderson, Stacey E. ; Mead BJ ; Munson AE
• Description:
  The plaque-forming cell (PFC) assay measures the humoral immune response mediated by the concerted actions of antigen-presenting cells, T lymphocytes, and B lymphocytes. The most common form of the plaque method is used for the detection of murine primary IgM antibodies directed against the T cell-dependent sheep red blood cell (sRBC) surface antigens. Research has shown that the PFC response to sRBC is not only an excellent monitor of the primary effector function of the B cell, but that it may be the most sensitive immune parameter currently available to identify chemical perturbation. Several modifications and variations of the PFC assay are described in detail in this unit. [Description provided by NIOSH]
• Subjects:
  Cells, Cultured Chemistry Communicable Diseases Epidemiology Immune System Occupational Health Risk Assessment Risk Factors Safety
• Keywords:
  Cell-culture-techniques Cell-function Chemical-properties Diseases Immune-system Immune-system-disorders Immunoglobulins Immunological-tests Immunotoxins Infectious-diseases Models Qualitative-analysis Quantitative-analysis Risk-analysis Risk-factors

  More +
• ISBN:
  9780471140856
• ISSN:
  1934-9254
• Publisher:
  John Wiley and Sons, Inc.
• Document Type:
  Text
• Genre:
  Book
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  2
• NIOSHTIC Number:
  nn:20033365
• Citation:
  Current protocols in toxicology. Costa LG, Hodgson E, Reed DJ, Maines MD, Sassa S, Sipes IG, Bradfield C, Lawrence DA, Morgan KS, eds. New York: Wiley and Sons, Inc., 2006 Aug; 2(Suppl 29):18.11.1-18.11.19
• Editor(s):
  Costa LG; Hodgson E; Reed DJ; Maines MD; Sassa S; Sipes IG; Bradfield C; Lawrence DA; Morgan KS
• Federal Fiscal Year:
  2006
• Peer Reviewed:
  False
• Source Full Name:
  Current protocols in toxicology
• Supplement:
  29
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:17ac78109ef15be5cc123acc59a8d17961d07763cab72bc408ff999d9b273a0d9aa397b452b90106aa3e53bce865ff9e2d09bb7d130d509821e397ffee6f39e8
• Download URL:
  https://stacks.cdc.gov/view/cdc/188979/cdc_188979_DS1.pdf
• File Type:
  [PDF - 250.28 KB ]