Application of Oligonucleotide Microarray Technology to Toxic Occupational Exposures

Details

• Personal Author:
  Gwinn MR ; Weston A
• Description:
  Microarray technology has advanced toward analysis of toxic occupational exposures in biological systems. Microarray analysis is an ideal way to search for biomarkers of exposure, even if no specific gene or pathway has been identified. Analysis may now be performed on thousands of genes simultaneously, as opposed to small numbers of genes as in the past. This ability has been put to use to analyze gene expression profiles of a variety of occupational toxins in animal models to classify toxins into specific categories based on response. Analysis of normal human cell strains allows an extension of this analysis to investigate the role of interindividual variation in response to various toxins. This methodology was used to analyze four occupationally related toxins in our lab: oxythioquinox (OTQ), a quinoxaline pesticide; malathion, an organophosphate pesticide; di-n-butyl phthalate (DBP), a chemical commonly found in personal care and cosmetic items; and benzo[a]pyrene (BaP), an environmental and occupational carcinogen. The results for each exposure highlighted signaling pathways involved in response to these occupational exposures. Both pesticides showed increase in metabolic enzymes, while DBP showed alterations in genes related to fertility. BaP exposure showed alterations in two cytochrome P450s related to carcinogenicity. When used with occupational exposure information, these data may be used to augment risk assessment to make the workplace safer for a greater proportion of the workforce, including individuals susceptible to disease related to exposures. [Description provided by NIOSH]
• Subjects:
  Biomarkers Chemistry Genetics Hazardous Substances Hypersensitivity Insecticides Neoplasms Occupational Exposure Occupational Health Risk Assessment Risk Factors Safety Toxicology Workplace

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• Keywords:
  Biological-monitoring Cancer Chemical-analysis Chemical-composition Chemical-hypersensitivity Chemical-properties Chemical-reactions Exposure-levels Exposure-methods Genes Occupational-exposure Pesticides-and-agricultural-chemicals Risk-analysis Risk-factors Toxicopathology Toxins Workplace-monitoring Workplace-studies

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• ISSN:
  1528-7394
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  315-324
• Volume:
  71
• Issue:
  5
• NIOSHTIC Number:
  nn:20033319
• Citation:
  J Toxicol Environ Health A 2008 Jan; 71(5):315-324
• Contact Point Address:
  Ainsley Weston, PhD, Toxicology and Molecular Biology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health/CDC, H2900, 1095 Willowdale Road, Morgantown, WV 26505
• Email:
  agw8@cdc.gov
• Federal Fiscal Year:
  2008
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Toxicology and Environmental Health, Part A: Current Issues
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0bbe6008ac582cf67a0a245beb3b5c949d1c05677dae61e51db2d0edb24a5edd762b80e2a6f5dda506456fbae0a8fbebcd4d5ecb5b22319473096487a7f12894
• Download URL:
  https://stacks.cdc.gov/view/cdc/188945/cdc_188945_DS1.pdf
• File Type:
  [PDF - 1.89 MB ]