Distribution of Injury and Microdosimetry of Ozone in the Ventilatory Unit of the Rat

Details

• Personal Author:
  Crapo JD ; Mercer RR ; Pinkerton KE ; Plopper CG
• Description:
  The distribution of ozone-induced injury across ventilatory units of the lungs was determined and compared with the predicted distribution of ozone dose across the same units to evaluate dose-response relationships. Sprague-Dawley rats were exposed to either 0.98 ppm ozone 8 h/day for 90 days or to filtered air only. En bloc microdissection was used to identify and isolate in longitudinal profile the bronchiole-alveolar duct junction, first pair of alveolar duct generations, and intervening bifurcation ridge. The first alveolar outpocketing along the bronchiolar wall of each isolation was used to identify the center of a series of concentric arcs radiating outward at 100-microns intervals across each ventilatory unit. The intercept lengths of each arc with the tissue of alveolar septal tips (edges) and alveolar walls were measured and expressed as a function of distance into the ventilatory unit. Relative ozone dose across the ventilatory unit was estimated using the geometry of the tracheobronchial tree and the volume and surface area distribution within individual ventilatory units. This mathematical model of ozone dose demonstrated a high degree of correlation to this measured tissue injury response. The findings of this study demonstrate that microdosimetry and microtoxicology can be used to determine dose-response relationships within the ventilatory unit and to assess questions of tissue sensitivity in ozone-induced lung injury. [Description provided by NIOSH]
• Subjects:
  Animals Chemistry Hypersensitivity Lung Lung Diseases Occupational Health Respiratory Function Tests Respiratory Protective Devices Respiratory System Respiratory Tract Diseases Safety Ventilation

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• Keywords:
  Animal-studies Author Keywords: Lung Bronchiole-alveolar Duct Junction Chemical-analysis Chemical-hypersensitivity Chemical-indicators Chemical-reactions Chemical-synthesis Dose Response En Bloc Microdissection Lung-burden Lung-disease Lung-disorders Lung-fibrosis Lung-function Lung-irritants Respirators Respiratory-function-tests

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• ISSN:
  8750-7587
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; North Carolina ; OSHA Region 4 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  73
• Issue:
  3
• NIOSHTIC Number:
  nn:20033131
• Citation:
  J Appl Physiol 1992 Sep; 73(3):817-824
• Contact Point Address:
  Kent E. Pinkerton, Department of Anatomy, School of Veterinary Medicine, University of California, Davis 95616
• Federal Fiscal Year:
  1992
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  19900930
• Source Full Name:
  Journal of Applied Physiology
• End Date:
  20020929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7592cc7edc1fd8123422b3adfa2d826ccf13ddb1500c59f75d95fd2236d96664e3151854d6c3a1876baf3f9365e92488591762b55cdfb8ff011a70dba9a97df0
• Download URL:
  https://stacks.cdc.gov/view/cdc/188824/cdc_188824_DS1.pdf
• File Type:
  [PDF - 18.56 MB ]