Diagnostic Assays for Crimean-Congo Hemorrhagic Fever

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Vanhomwegen, Jessica ; Alves, Maria João ; Županc, Tatjana Avšič ; Bino, Silvia ; Chinikar, Sadegh ; Karlberg, Helen ; Korukluoğlu, Gülay ; Korva, Miša ; Mardani, Masoud ; Mirazimi, Ali ; Mousavi, Mehrdad ; Papa, Anna ; Saksida, Ana ; Sharifi-Mood, Batool ; Sidira, Persofoni ; Tsergouli, Katerina ; Wölfel, Roman ; Zeller, Hervé ; Dubois, Philippe
• Description:
  Crimean-Congo hemorrhagic fever (CCHF) is a highly contagious viral tick-borne disease with case-fatality rates as high as 50%. We describe a collaborative evaluation of the characteristics, performance, and on-site applicability of serologic and molecular assays for diagnosis of CCHF. We evaluated ELISA, immunofluorescence, quantitative reverse transcription PCR, and low-density macroarray assays for detection of CCHF virus using precharacterized archived patient serum samples. Compared with results of local, in-house methods, test sensitivities were 87.8%-93.9% for IgM serology, 80.4%-86.1% for IgG serology, and 79.6%-83.3% for genome detection. Specificity was excellent for all assays; molecular test results were influenced by patient country of origin. Our findings demonstrate that well-characterized, reliable tools are available for CCHF diagnosis and surveillance. The on-site use of such assays by health laboratories would greatly diminish the time, costs, and risks posed by the handling, packaging, and shipping of highly infectious biologic material.
• Subjects:
  CCHF CCHFV Crimean-Congo Hemorrhagic Fever Diagnosis Diagnostics ELISA Enzyme-Linked Immunosorbent Assay Hemorrhagic Fever, Crimean Hemorrhagic Fever Virus, Crimean-Congo Humans IFA Immunofluorescent Assay Microarray Analysis Molecular Multicenter PCR Polymerase Chain Reaction QRT-PCR Reagent Kits, Diagnostic Reproducibility Of Results Research Reverse Transcription PCR Sensitivity And Specificity Serologic Tests Serology Tick-borne Infections Vector-borne Infections Viruses

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• Source:
  Emerg Infect Dis. 18(12):1958-1965.
• Document Type:
  Journal Article
• Volume:
  18
• Issue:
  12
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:bbc05fbb30573e4120f06b8cb959f17fbc0e1d1ddbd6017e0bfdc25c8b90db78
• Download URL:
  https://stacks.cdc.gov/view/cdc/18876/cdc_18876_DS1.pdf
• File Type:
  [PDF - 315.59 KB ]