Target Size of Neurotoxic Esterase and Acetylcholinesterase as Determined by Radiation Inactivation

Details

• Personal Author:
  Abou-Donia MB ; Carrington CD ; Fluke DJ
• Description:
  The target size of neurotoxic esterase (NTE), the putative target site for the initiation of organophosphorus-compound-induced delayed neurotoxicity, and acetylcholinesterase (AChE) from hen brain were examined by determining the rate at which the activities of the esterases were destroyed by ionizing irradiation. Samples of hen brain were prepared by slowly drying a microsomal preparation under vacuum. The dried samples were then irradiated with electrons from a 1 MeV Van de Graaff generator. The doses ranged from 0 to 28 Mrad. The radiation doses were calibrated by the rate of inactivation of T1-bacteriophage plaque induction. Following the irradiation procedure, the samples were resuspended in buffer and enzymic activity was measured. The target size of NTE from hen brain was determined to be about 105 kDa, whereas hen brain AChE was found to have a target size of about 53 kDa. The target size of NTE was found to be similar in experiments with rat brain and cat brain. In addition, commercial preparations of electric-eel electric-organ AChE and horse serum butyrylcholinesterase were found to have target sizes that were identical with each other, and also were very similar to that of AChE from hen brain. [Description provided by NIOSH]
• Subjects:
  Animals Environmental Monitoring Laboratories Nervous System Occupational Health Radiation Safety
• Keywords:
  Animal-studies Brain-function Central-nervous-system Exposure-assessment Exposure-levels Ionizing-radiation Irradiation Laboratory-animals Neurotoxic-effects Neurotoxicity
• ISSN:
  0264-6021
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  231
• Issue:
  3
• NIOSHTIC Number:
  nn:20037451
• Citation:
  Biochemical J 1985 Nov; 231(3):789-792
• Contact Point Address:
  Mohamed B. Abou-Donia, Department of Pharmacology, Duke University Medical Center, Durham, NC 27710, U.S.A.
• Federal Fiscal Year:
  1986
• Performing Organization:
  Duke University, Durham, North Carolina
• Peer Reviewed:
  True
• Start Date:
  19840601
• Source Full Name:
  Biochemical Journal
• End Date:
  19881031
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c96d9643e8c0fef7bfd380fd906430b352ce91670ecb6ca44a992a449c495e3493595dab244756692c34d2c1573706f6d9cc4f0d150c9c446b584faa361cdcf2
• Download URL:
  https://stacks.cdc.gov/view/cdc/188546/cdc_188546_DS1.pdf
• File Type:
  [PDF - 606.48 KB ]