Evaluation of Dicarbonyls Generated in a Simulated Indoor Air Environment Using an In Vitro Exposure System

Details

• Personal Author:
  Anderson, Stacey E. ; Franko J ; Jackson LG ; Wells JR
• Description:
  Over the last two decades, there has been increasing awareness regarding the potential impact of indoor air pollution on health. Exposure to volatile organic compounds (VOCs) or oxygenated organic compounds formed from indoor chemistry has been suggested to contribute to adverse health effects. These studies use an in vitro monitoring system called VitroCell, to assess chemicals found in the indoor air environment. The structurally similar dicarbonyls diacetyl, 4-oxopentanal (4-OPA), glyoxal, glutaraldehyde, and methyl glyoxal were selected for use in this system. The VitroCell module was used to determine whether these dicarbonyls were capable of inducing inflammatory cytokine expression by exposed pulmonary epithelial cells (A549). Increases in the relative fold change in messenger RNA expression of the inflammatory mediators, interleukin (IL)-6, IL-8, granulocyte-macrophage colony-stimulating factor (GM-CSF), and tumor necrosis factor alpha (TNF-a) were identified following exposure to diacetyl, 4-OPA, glyoxal, glutaraldehyde, and methyl glyoxal when compared to a clean air control. Consistent results were observed when the protein levels of these cytokines were analyzed. Exposure to 4-OPA significantly elevated IL-8, IL-6, GM-CSF, and TNF-a while glutaraldehyde caused significant elevations in IL-6, IL-8, and TNF-a. IL-6 and IL-8 were also significantly elevated after exposure to diacetyl, glyoxal, and methyl glyoxal. These studies suggest that exposure to structurally similar oxygenated reaction products may be contributing to some of the health effects associated with indoor environments and may provide an in vitro method for identification and characterization of these potential hazards. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Air Pollution Air Pollution, Indoor Biochemistry Chemistry Cytology Environmental Monitoring Hazardous Substances Hypersensitivity Lung Occupational Health Respiratory System Risk Assessment Risk Factors Safety Volatile Organic Compounds Workplace

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• Keywords:
  Air-contamination Air-monitoring Air-quality Air-quality-measurement Air-quality-monitoring Author Keywords: Dicarbonyls Biochemical-tests Biodynamics Biological-effects Biological-monitoring Biostatistics Cell-biology Cell-metabolism Cellular-reactions Chemical-composition Chemical-hypersensitivity Chemical-properties Chemical-reactions Exposure-assessment Exposure-levels Exposure-methods Indoor Air Inflammatory Cytokines Organic-vapors Pulmonary-system Risk-analysis Risk-factors Statistical-analysis Toxic-effects Toxic-materials VitroCell VOC Work-environment Workplace-studies

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  453-461
• Volume:
  115
• Issue:
  2
• NIOSHTIC Number:
  nn:20036866
• Citation:
  Toxicol Sci 2010 Feb; 115(2):453-461
• Contact Point Address:
  Stacey E. Anderson, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Drive, Morgantown, WV 26505
• Email:
  sanderson4@cdc.gov
• CAS Registry Number:
  Butanedione (CAS RN 431-03-8) ; Glutaraldehyde (CAS RN 111-30-8) ; Glyoxal (CAS RN 107-22-2) ; Methylglyoxal (CAS RN 78-98-8)
• Federal Fiscal Year:
  2010
• NORA Priority Area:
  Healthcare and Social Assistance ; Services
• Peer Reviewed:
  True
• Source Full Name:
  Toxicological Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:32d61fe420b2202c0efee2f3ce6754a7567c8b53948f49ced25db9fa0fa6253d0eb0d69febf1cd849834598333e6bcaeb636e4bf894b97dd624ced76e9327509
• Download URL:
  https://stacks.cdc.gov/view/cdc/188112/cdc_188112_DS1.pdf
• File Type:
  [PDF - 366.85 KB ]