Oxidative Injury in the Lungs of Neonatal Rats Following Short-Term Exposure to Ultrafine Iron and Soot Particles

Details

• Personal Author:
  Aust AE ; Chen C-Y ; Kennedy IM ; Pinkerton KE ; Smith KR ; Zhong C-Y ; Zhou Y-M
• Description:
  Greater risk of adverse effects from particulate matter (PM) has been noted in susceptible subpopulations, such as children. However, the physicochemical components responsible for these biological effects are not understood. As critical constituents of PM, transition metals were postulated to be involved in a number of pathological processes of the respiratory system through free radical-medicated damage. The purpose of this study was to examine whether oxidative injury in the lungs of neonatal rats could be induced by repeated short-term exposure to iron (Fe) and soot particles. Sprague Dawley rats 10 d of age were exposed by inhalation to two different concentrations of ultrafine iron particles (30 or 100 microg/m(3)) in combination with soot particles adjusted to maintain a total particle concentration of 250 microg/m(3). Exposure at 10 d and again at 23 d of age was for 6 h/d for 3 d. Oxidative stress was observed at both Fe concentrations in the form of significant elevations in glutathione disulfide (GSSG) and GSSG/glutathione (GSH) ratio and a reduction in ferric/reducing antioxidant power in bronchoalveolar lavage. A significant decrease in cell viability associated with significant increases in lactate dehydrogenase (LDH) activity, interleukin-1-beta (IL-1beta), and ferritin expression was noted following exposure to particles containing the highest Fe concentration. Iron from these particles was shown to be bioavailable in an in vitro assay using the physiologically relevant chelator, citrate. Data indicate that combined Fe and soot particle exposure induces oxidative injury, cytotoxicity and pro-inflammatory responses in the lungs of neonatal rats. [Description provided by NIOSH]
• Subjects:
  Biochemistry Cytotoxins Dust Laboratories Lung Occupational Health Particulate Matter Respiratory System Safety
• Keywords:
  Biochemical Analysis Biodynamics Cellular Reactions Cytotoxic Effects Exposure Assessment Inhalation Studies Laboratory-animals Lung Irritants Particulate Dust Particulates
• ISSN:
  1528-7394
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  73
• Issue:
  12
• NIOSHTIC Number:
  nn:20036717
• Citation:
  J Toxicol Environ Health A 2010 Jan; 73(12):837-847
• Contact Point Address:
  Dr. Kent E. Pinkerton, Center for Health and the Environment, Old Davis Road, University of California, Davis, CA 95616
• Email:
  kepinkerton@ucdavis.edu
• CAS Registry Number:
  Iron (CAS RN 7439-89-6)
• Federal Fiscal Year:
  2010
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Journal of Toxicology and Environmental Health, Part A: Current Issues
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d342d49d9fa80dbb45cd0630751f37eb56168a5c1ddf1f00f1c62076fc9b8a5d0eff496cfd450ad259d7d68b6ba7cefb05e83e2ce7d6427682ea2f6e8b383585
• Download URL:
  https://stacks.cdc.gov/view/cdc/188027/cdc_188027_DS1.pdf
• File Type:
  [PDF - 443.25 KB ]