Development of a Modified Glucan-Specific Limulus Amebocyte Lysate Method Which Correlates Murine Pulmonary Inflammation Induced by Floor Dust Collected from a Water-Damaged Building

Details

• Personal Author:
  Antonini JM ; Castranova, Vincent ; Cox-Ganser, Jean M. ; Li S ; Park J ; Shogren ES ; Wolfarth MG ; Young S
• Description:
  1C3-beta-glucan, a major cell wall component of fungi, induces pulmonary inflammation. There is inconsistency in correlation between the levels of glucan measured by current detection methods and the respiratory inflammation observed in people or laboratory animals exposed to fungi. There is a need for a method that better assesses the inflammatory potential of 1C3-beta-glucans in environmental samples. We used the glucan-specific (G-specific) Limulus Amebocyte Lysate (LAL) method after extraction with dimethyl sulfoxide (DMSO), sodium hydroxide (NaOH), or water to analyze the glucan content in floor dusts from a water-damaged building. C3HeB/FeJ mice, an endotoxin-sensitive strain, were treated with different dusts (2.5 mg/kg of body weight), or saline (vehicle control) by pharyngeal aspiration. At 18 hr after aspiration, bronchoalveolar lavage (BAL) was performed, and lung inflammation and injury were assessed by measuring: (1) neutrophil (PMN) infiltration, (2) inflammatory cytokines (IL-6, IL-10, MCP-1, IFN-y, TNF-alpha, and IL12- p70) levels and (3) albumin and lactate dehydrogenase in recovered BAL fluid. Both DMSO and NaOH extaction increased the detection of glucan by approximately20 fold compared to water extraction. Only the DMSO extraction method showed a statistically significant correlation (p<0.05) between 1C3-beta-glucan levels and albumin, total BAL cells, PMNs recovered, TNF-alpha, MCP-1, and IL-6. As expected, significant positive correlations (p<0.05) were also found comparing endotoxin levels and BAL cell numbers, PMNs recovered, IL-6, IFN-y and MCP-1. In conclusion, DMSO extraction for glucan analysis may prove useful in understanding the impact of environmental contamination of glucans on lung disease. [Description provided by NIOSH]
• Subjects:
  Dust Endotoxins Environmental Exposure Environmental Monitoring Environmental Pollution Fungi Hazardous Substances Irritants Laboratories Lung Lung Diseases Occupational Health Particulate Matter Respiratory Hypersensitivity Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Biological-effects Cell-biology Cell-damage Cell-function Cellular-reactions Dust-exposure Dust-particles Environmental-exposure Environmental-pollution Exposure-assessment Exposure-levels Exposure-methods Fungal-diseases Fungal-infections Inhalation-studies Laboratory-animals Laboratory-testing Lung-cells Lung-disorders Lung-function Lung-irritants Particle-aerodynamics Particulate-dust Pulmonary-disorders Pulmonary-system-disorders Respiratory-hypersensitivity Respiratory-infections Respiratory-irritants Statistical-analysis Toxic-effects

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; OSHA Region 8 ; Utah ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  114
• Issue:
  1
• NIOSHTIC Number:
  nn:20036616
• Citation:
  Toxicologist 2010 Mar; 114(1):157
• Federal Fiscal Year:
  2010
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 49th Annual Meeting and ToxExpo, March 7-11, 2010, Salt Lake City, Utah
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b42f529e0d5ef0deecc5212e7cf2666080bdb9a312aee44ca831d529ad897b7bd2000162a0de39a0c8bf64a0cdd883d156618fa04e819b5348aa9009e2050578
• Download URL:
  https://stacks.cdc.gov/view/cdc/187970/cdc_187970_DS1.pdf
• File Type:
  [PDF - 732.67 KB ]