Effect of Inhalation of Gas Metal ARC Stainless Steel Welding Fume on Mouse Lung Inflammation and Tumorigenesis

Details

• Personal Author:
  Antonini JM ; Battelli LA ; Chen BT ; Erdely A ; Frazer DG ; Kashon ML ; Stone S ; Young S ; Zeidler-Erdely PC
• Description:
  Previously, we found strain dependent differences in the degree and resolution of the lung response in tumor susceptible (A/J) and resistant (C57BL/6J) mice exposed by pharyngeal aspiration to stainless steel (SS) welding fumes. We also found a borderline significant tumorigenic effect of gas metal arc (GMA)-SS fume. In this study, our objective was to further examine the inflammatory and potential carcinogenic effects of GMA-SS fume in mice via an inhalation exposure route. Male A/J and C57BL/6J mice were exposed to GMA-SS welding fume (40 mg/m3 x 3 hr/day x 6 or 10 days) or filtered air. At day 1, 4, 7, 10, 14 and 28 after the last exposure day, bronchoalveolar lavage (BAL) was done postmortem. Lung cytotoxicity (lactate dehydrogenase activity), air-blood barrier damage (albumin), inflammatory cytokines (IFN-y, IL-6, IL-10, IL-12p70, MCP-1, MIP-2, TNF-alpha), total BAL cell counts and differentials (.300 cells identified) were analyzed. Gross tumor counts and sizes were assessed in A/J mice at 78 weeks post-exposure. In both strains, inhalation of SS fume caused significant cytotoxicity and damage at all time points compared to air. Also, an early sustained macrophage and lymphocyte influx was found followed by a gradual neutrophil accumulation with no resolution of the response at 28 days post-exposure. MCP-1, MIP-2 and TNF-á were increased in both strains while the C57BL/6J also had increased IL-6 BAL protein. Tumor incidence and multiplicity in the 6 and 10 day GMA-SS-exposed groups were not significantly different from the corresponding air control groups. Furthermore, no tumor size differences were found in this study. Overall, inhalation of GMA-SS welding fume caused a similar lung inflammatory response in both mouse strains that was unresolved at 28 days post-exposure. Additionally, under the experimental conditions, GMA-SS welding fume did not significantly affect tumor development in A/J mice. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Carcinogens Cytotoxins Environmental Monitoring Hazardous Substances Irritants Laboratories Lung Lung Diseases Neoplasms Occupational Health Respiratory System Respiratory Tract Diseases Safety Welding

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• Keywords:
  Biological-effects Carcinogenicity Cell-biology Cell-damage Cellular-reactions Cytotoxic-effects Exposure-assessment Exposure-levels Exposure-methods Fumes Gas-sampling Gas-welders Inhalation-studies Laboratory-animals Laboratory-testing Lung-cells Lung-function Pulmonary-cancer Pulmonary-system-disorders Respiratory-irritants Statistical-analysis Toxic-effects Welders-lung

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; OSHA Region 8 ; Utah ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  114
• Issue:
  1
• NIOSHTIC Number:
  nn:20036615
• Citation:
  Toxicologist 2010 Mar; 114(1):156
• Federal Fiscal Year:
  2010
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 49th Annual Meeting and ToxExpo, March 7-11, 2010, Salt Lake City, Utah
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:29d02ff8bdf5311fa8e96cfd4f95ef5d00d594571ea985dd1219fcdd7de7b4788f2a35df9197e55c104ae55a0189db121cb2cfd6d3b9ec01ad5fdf6af7e65f5e
• Download URL:
  https://stacks.cdc.gov/view/cdc/187969/cdc_187969_DS1.pdf
• File Type:
  [PDF - 731.83 KB ]