Effects of Glutathione Depletion and Age on Skeletal Muscle Performance and Morphology Following Chronic Stretch-Shortening Contraction Exposure

Details

• Personal Author:
  Baker BA ; Cutlip, Robert G. ; Hollander MS ; Kashon ML
• Description:
  The involvement of glutathione in the response of skeletal muscle following repetitive, high-intensity mechanical loading is not known. We examined the influence of a glutathione antagonist [l-Buthionine Sulfoximine (BSO)] had on the adaptability of skeletal muscle during chronic mechanical loading via stretch-shortening contractions (SSCs) in young and old rats. Left dorsiflexor muscles of young (12 weeks, N = 16) and old (30 months, N = 16), vehicle- and BSO-treated rats were exposed three times per week for 4.5-weeks to a protocol of 80 maximal SSCs per exposure in vivo. Skeletal muscle response to the SSC exposure was characterized by muscle performance, as well as muscle wet-weight and quantitative morphological analyses following the exposure period. Results reveal that generally, muscle performance increased in the young rats only following chronic SSC exposure. BSO treatment had no effect on muscle performance or morphology following the chronic SSC exposure in old rats. Muscle wet-weight was increased following exposure compared with the contra-lateral control limb, irrespective of age (p < 0.05). Muscle cross-sectional area increased approximately 20% with SSC loading in the young, vehicle rats, while increasing approximately 10% with SSC loading in old, vehicle rats compared with control rat muscle. No degenerative myofibers were noted in either age group, but edema were increased as a result of aging (p < 0.05). We conclude that our results indicate that glutathione depletion does not adversely affect muscle performance or morphology in old rats. Nevertheless, we continue to show that aging negatively influences performance and morphology following chronic SSC exposure. [Description provided by NIOSH]
• Subjects:
  Age Factors Aging Biomechanical Phenomena Environmental Monitoring Laboratories Musculoskeletal Diseases Musculoskeletal System Occupational Health Oxidants Safety
• Keywords:
  Age-factors Author Keywords: Adaptation Biological-monitoring Biomechanics Exposure-assessment Exposure-levels Exposure-methods Glutathione Inflammation Laboratory-animals Laboratory-testing Muscle-function Muscle-physiology Muscle-stress Muscle Hypertrophy Muscles Musculoskeletal-system Musculoskeletal Disorders Oxidative-metabolism Physiological-factors Stretch-shortening Contractions

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• ISSN:
  1439-6319
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  108
• Issue:
  3
• NIOSHTIC Number:
  nn:20036418
• Citation:
  Eur J Appl Physiol 2010 Feb; 108(3):619-630
• Contact Point Address:
  Robert G. Cutlip, National Institute for Occupational Safety and Health (NIOSH), Health Effects Laboratory Division, TMBB, 1095 Willowdale Road, M-S 2027, Morgantown, WV 26505
• Email:
  rgc8@cdc.gov
• Federal Fiscal Year:
  2010
• Peer Reviewed:
  True
• Source Full Name:
  European Journal of Applied Physiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:af9c34ad7f224d71866f8ec02df28e69425f147cd8c39603d18c4eb7bcefca364fe27c5f37c3bf2de8566d0a17a5d11eae738dcf51c750f82125fe4155cc327c
• Download URL:
  https://stacks.cdc.gov/view/cdc/187859/cdc_187859_DS1.pdf
• File Type:
  [PDF - 2.33 MB ]