Effects of Age and Glutathione Levels on Oxidative Stress in Rats After Chronic Exposure to Stretch-Shortening Contractions

Details

• Personal Author:
  Baker BA ; Cutlip, Robert G. ; Ensey J ; Hollander MS ; Kashon ML
• Description:
  We investigated effects of age and glutathione synthesis inhibition on the oxidative stress status of tibialis anterior muscles from young and old Fisher 344 x Brown Norway male rats after chronic administration of stretch-shortening contractions. Oral supplementation of l-buthionine-(S,R)-sulfoximine (BSO) inhibited glutathione synthesis. Dorsiflexor muscles in the hindlimb were exposed to 80 maximal stretch-shortening contractions (SSCs) three times per week for 4.5 weeks. We measured malondialdehyde, hydrogen peroxide (H2O2), and free isoprostanes to determine oxidative stress. Glutathione peroxidase activity was measured as an indicator of H2O2 scavenging. Glutathione measurements confirmed the effectiveness of BSO treatment. In young rats, the SSC exposure protocol prevented oxidative stress and enhanced H2O2 scavenging. In old rats, malondialdehyde was increased in the exposed muscle and a BSO-induced increase in H2O2 was not alleviated with SSC exposure as seen in young rats. In addition, glutathione peroxidase activity and total glutathione were increased in old rats relative to their young counterparts. All comparisons were significant at the 0.05 level. Overall, BSO administration was effective in decreasing total glutathione levels and increasing H2O2 levels in old and young rats exposed to SSCs. In addition, effects of chronic exposure to high-force resistive loading SSCs in active muscle from old animals are: (1) antioxidant capacity is enhanced similar to what is seen with endurance training and (2) oxidative stress is increased, probably as a consequence of the enhanced vulnerability due to aging. [Description provided by NIOSH]
• Subjects:
  Biomechanical Phenomena Environmental Monitoring Laboratories Musculoskeletal Diseases Musculoskeletal System Occupational Health Oxidants Safety
• Keywords:
  Author Keywords: Aging Biological-monitoring Biomechanics Exposure-assessment Exposure-levels Exposure-methods In Vivo L-Buthionine-(S,R)-sulfoximine Laboratory-animals Laboratory-testing Muscle Muscle-function Muscle-physiology Muscle-stress Muscles Musculoskeletal-system Musculoskeletal Disorders Oxidative-metabolism Oxidative Stress Physiological-factors Stretch-shortening Contraction

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• ISSN:
  1439-6319
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  108
• Issue:
  3
• NIOSHTIC Number:
  nn:20036417
• Citation:
  Eur J Appl Physiol 2010 Feb; 108(3):589-597
• Contact Point Address:
  Robert G. Cutlip, National Institute for Occupational Safety and Health (NIOSH), Health Effects Laboratory Division, TMBB, 1095 Willowdale Rd,M-S 3014, Morgantown, WV 26505
• Email:
  rgc8@cdc.gov
• Federal Fiscal Year:
  2010
• Peer Reviewed:
  True
• Source Full Name:
  European Journal of Applied Physiology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ee3acfcbe57cafd70029b4f38d3853b574a28ab1af88c53019e48b217ae3beaf2e840102f8eea8e2807fe1e46459b7529f33ab91da33a7d0479934bc12fb8990
• Download URL:
  https://stacks.cdc.gov/view/cdc/187858/cdc_187858_DS1.pdf
• File Type:
  [PDF - 451.92 KB ]