Hog Confinement Dust Enhances Neutrophil Adherence to Airway Epithelial Cells

Details

• Personal Author:
  Heires AJ ; Hilger MA ; Romberger DJ ; Von Essen SG ; Wyatt TA
• Description:
  Rationale: Persons exposed to swine confinement facilities have evidence of neutrophilic airway inflammation. We previously reported that dust extract from swine facilities is a potent stimulant of interleukin-8 (IL-8) from airway epithelial cells in vitro that is mediated by protein kinase C (PKC) isoforms alpha and epsilon. We hypothesized that dust extract also modulates the ability of neutrophils to adhere to airway epithelium. Methods: Confluent human bronchial epithelial cell monolayers (BEAS-2B) were pre-treated with one of the following agents: anti-ICAM-1 neutralizing antibody or PKC-a or -e specific inhibitors (Gö6976, RO-318220, respectively) before being exposed to 5% hog confinement dust extract (HDE) for up to 24h. Two epithelial cell lines with inactive PKCa or PKCe (PKCaDN, PKCeDN respectively) were also used. Fresh blood neutrophils (PMN, 300x105/well) labeled with the fluorescent dye calcein-AM were allowed to adhere to the BEAS-2B for 30 minutes. Adherent cells were quantified photometrically. Six wells were averaged for each condition; experiments were repeated 3 times. Multiple comparisons were analyzed using ANOVA with Bonferroni posttest. Results: Adhesion of PMN to BEAS-2B cells treated with HDE was increased in both a time- and dose-dependent fashion. Inhibition of BEAS-2B PKCa using Gö6976 or the PKCaDN cell line resulted in a 55.4%, and 51.4% reduction in PMN adherence, respectively (p<.01). Likewise, PKCe inhibition by RO-318220 or PKCeDN cells resulted in a 55.8% and 58.1% decrease, respectively (p<.05). Neutralizing antibody blockade of ICAM-1 (1:50 dilution) reduced PMN adherence dose-responsively (68.63%, p<.01). Conclusions: Dust extract mediates PMN adherence predominantly by influencing the epithelium and involves PKC isoforms a and e and ICAM-1. [Description provided by NIOSH]
• Subjects:
  Biological Factors Dust Environmental Monitoring Irritants Lung Lung Diseases Occupational Health Respiratory Hypersensitivity Respiratory System Respiratory Tract Diseases Safety Workplace

  More +
• Keywords:
  Biological-factors Cell-biology Cell-function Dust-exposure Dust-inhalation Exposure-assessment Exposure-levels Exposure-methods Inhalation-studies Lung-disorders Lung-function Lung-irritants Mathematical-models Physiological-effects Physiological-measurements Pulmonary-system-disorders Respiratory-hypersensitivity Respiratory-irritants Respiratory-system-disorders Statistical-analysis Work-environment Workplace-studies

  More +
• ISSN:
  1073-449X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  Nebraska ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  179
• NIOSHTIC Number:
  nn:20036384
• Citation:
  Am J Respir Crit Care Med 2009 Apr; 179(Meeting Abstracts):A2197
• Email:
  aheires@unmc.edu
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center, Omaha, Nebraska
• Peer Reviewed:
  False
• Start Date:
  20060801
• Source Full Name:
  American Journal of Respiratory and Critical Care Medicine
• Supplement:
  Meeting Abstracts
• End Date:
  20160731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:88089fa297b3052233b6bf9fc88fdcb6904b3320ccddc396eede3a227292a6ea9e2caa3477341bb4f12e0caaf919c66f9a65b056dcb2c1767b8411e29eb1bb0d
• Download URL:
  https://stacks.cdc.gov/view/cdc/187841/cdc_187841_DS1.pdf
• File Type:
  [PDF - 146.27 KB ]