Organic Dust Exposure Alters Monocyte-Derived Dendritic Cell Differentiation and Maturation

Details

• Personal Author:
  Alexis NE ; Burrell AM ; Parks C ; Poole JA ; Romberger DJ ; Thiele GM
• Description:
  Organic dust exposure in agricultural animal environments results in airway diseases. Dendritic cells (DCs) orchestrate inflammatory immune response in the airways, but little is known about how organic dust affects differentiation and maturation of monocyte-derived immature and mature DCs (iDCs, mDCs). Peripheral blood monocytes were differentiated in vitro into iDCs with granulocyte-macrophage colony stimulating factor + IL-4 (6 days) with and without swine facility organic dust extract (ODE, 0.1%). Unlike control iDCs, ODE-conditioned iDCs maintained key monocyte properties (increased mCD14, increased phagocytic ability) while expressing DC features [increased mCD83, HLA-DR, CD80, CD86, diminished cytokine (TNF-alpha, IL-6) responsiveness]. At day 6, iDCs were cultured for an additional 48 h (days 7 and 8) with lipopolysaccharide (LPS) to induce mDCs. ODE-conditioned mDCs maintained high expression of mCD14(+) and elevated phagocytosis while their DC features weakened as evidenced by decreased CD11c, CD83, HLA-DR, CD86, and CCR7 expression and reduced lymphocyte-stimulating capacity. Similar results were observed when monocytes were exposed to ODE for only the first 48 h and with ODE depleted of endotoxin. Control iDCs exposed to ODE during the final 2 days of iDC maturation (days 7 and 8) did not differ from control (no ODE) iDCs in surface marker expression and phagocytic ability, but exhibited enhanced lymphocyte-stimulating capacity. Dust exposure alters monocyte differentiation to iDCs and prevents maturation of iDC to mDCs. The first 48 h of monocyte differentiation appears to be the susceptible period to exposure. Environmental exposures present during early monocyte differentiation may impact the critical balance of DCs in the lung. [Description provided by NIOSH]
• Subjects:
  Agriculture Animal Husbandry Animals Asthma Asthma, Occupational Dust Irritants Lung Lung Diseases Occupational Exposure Occupational Health Respiratory System Respiratory Tract Diseases Safety Safety Management Workplace

  More +
• Keywords:
  Agricultural-industry Agricultural-processes Agricultural-workers Animal-husbandry-workers Author Keywords: Phagocytosis Bronchial-asthma Cell-biology Cell-function Cytokines Dust-particles Dusts Inhalation-studies Innate Cell Surface Molecules Lung-burden Lung-cells Lung-disorders Lung-function Lung-irritants Lymphocyte Proliferation Occupational-hazards Occupational-health Occupational-respiratory-disease Pulmonary-system Pulmonary-system-disorders Respiratory-irritants Respiratory-system-disorders Safety-measures Statistical-analysis Swine Work-environment Worker-health

  More +
• ISSN:
  1040-0605
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Nebraska ; North Carolina ; OSHA Region 4 ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  297
• Issue:
  4
• NIOSHTIC Number:
  nn:20036073
• Citation:
  Am J Physiol Lung Cell Mol Physiol 2009 Oct; 297(4):L767-L776
• Contact Point Address:
  J. A. Poole, Pulmonary, Critical Care, Sleep, and Allergy Section, University of Nebraska Medical Center, 985300 The Nebraska Medical Center, Omaha, NE 68198-5300
• Email:
  japoole@unmc.edu
• Federal Fiscal Year:
  2010
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center, Omaha, Nebraska
• Peer Reviewed:
  True
• Start Date:
  20060801
• Source Full Name:
  American Journal of Physiology: Lung Cellular and Molecular Physiology
• End Date:
  20160731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7171e80d49a0e41120b040b2602bfc81a11d6dd67972013d2f2d7daf31ab6a8b6f0470eaa553be202922118bf773b4e1ee7c2a2ebfb3cf260a3ee921cb5cab1f
• Download URL:
  https://stacks.cdc.gov/view/cdc/187667/cdc_187667_DS1.pdf
• File Type:
  [PDF - 1.33 MB ]