Hog Barn Dust Slows Airway Epithelial Cell Migration In Vitro Through a PKCalpha-Dependent Mechanism

Details

• Personal Author:
  Allen-Gipson DS ; DeVasure J ; Heires A ; Romberger DJ ; Sammut A ; Slager RE ; Von Essen, Susanna ; Wyatt TA
• Description:
  Agricultural work and other occupational exposures are responsible for approximately 15% of chronic obstructive pulmonary disease (COPD). COPD involves airway remodeling in response to chronic lung inflammatory events and altered airway repair mechanisms. However, the effect of agricultural dust exposure on signaling pathways that regulate airway injury and repair has not been well characterized. A key step in this process is migration of airway cells to restore epithelial integrity. We have previously shown that agents that activate the critical regulatory enzyme protein kinase C (PKC) slow cell migration during wound repair. Based on this observation and direct kinase measurements that demonstrate that dust extract from hog confinement barns (HDE) specifically activates the PKC isoforms PKCalpha and PKCepsilon, we hypothesized that HDE would slow wound closure time in airway epithelial cells. We utilized the human bronchial epithelial cell line BEAS-2B and transfected BEAS-2B cell lines that express dominant negative (DN) forms of PKC isoforms to demonstrate that HDE slows wound closure in BEAS-2B and PKCepsilon DN cell lines. However, in PKCalpha DN cells, wound closure following HDE treatment is not significantly different than media-treated cells. These results suggest that the PKCalpha isoform is an important regulator of cell migration in response to agricultural dust exposure. [Description provided by NIOSH]
• Subjects:
  Agriculture Asthma Asthma, Occupational Dust Lung Occupational Exposure Occupational Health Respiratory System Respiratory Tract Diseases Safety Safety Management Workplace

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• Keywords:
  Agricultural-workers Bronchial-asthma Cell-biology Cell-function Cell-migration Dust-particles Dusts Lung-burden Lung-cells Lung-irritants Occupational-hazards Pulmonary-system-disorders Respiratory-system-disorders Safety-measures Work-environment Worker-health

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• ISSN:
  1040-0605
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Nebraska ; OSHA Region 7
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  293
• Issue:
  6
• NIOSHTIC Number:
  nn:20033205
• Citation:
  Am J Physiol Lung Cell Mol Physiol 2007 Dec; 293(6):L1469-L1474
• Contact Point Address:
  T. A. Wyatt, Deptartment of Internal Medicine, Pulmonary and Critical Care Medicine Section, Univ. of Nebraska Medical Center, 985300 Nebraska Medical Center, Omaha, NE 68198-5300
• Email:
  twyatt@unmc.edu
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of Nebraska Medical Center, Omaha, Nebraska
• Peer Reviewed:
  True
• Start Date:
  20060801
• Source Full Name:
  American Journal of Physiology: Lung Cellular and Molecular Physiology
• End Date:
  20160731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e99f8b4bd0d9f9d838a277d25a6ed964217790862d4745c50b4fd6c88a09587084f5a0296fa7702e26a8b6b8de5b1e89c75fed2cca81092a6cc3defa834ed3b6
• Download URL:
  https://stacks.cdc.gov/view/cdc/188873/cdc_188873_DS1.pdf
• File Type:
  [PDF - 184.31 KB ]