Induction of Aneuploidy by Single-Walled Carbon Nanotubes

Details

• Personal Author:
  Battelli L ; Benkovic SA ; Friend S ; Hubbs, Ann F. ; Kashon ML ; Kisin ER ; Lowry DT ; McKinstry KT ; Murray AR ; Reynolds SH ; Salisbury JL ; Sargent LM ; Shvedova AA
• Description:
  Engineered carbon nanotubes are newly emerging manufactured particles with potential applications in electronics, computers, aerospace, and medicine. The low density and small size of these biologically persistent particles makes respiratory exposures to workers likely during the production or use of commercial products. The narrow diameter and great length of single-walled carbon nanotubes (SWCNT) suggest the potential to interact with critical biological structures. To examine the potential of nanotubes to induce genetic damage in normal lung cells, cultured primary and immortalized human airway epithelial cells were exposed to SWCNT or a positive control, vanadium pentoxide. After 24 hr of exposure to either SWCNT or vanadium pentoxide, fragmented centrosomes, multiple mitotic spindle poles, anaphase bridges, and aneuploid chromosome number were observed. Confocal microscopy demonstrated nanotubes within the nucleus that were in association with cellular and mitotic tubulin as well as the chromatin. Our results are the first to report disruption of the mitotic spindle by SWCNT. The nanotube bundles are similar to the size of microtubules that form the mitotic spindle and may be incorporated into the mitotic spindle apparatus. [Description provided by NIOSH]
• Subjects:
  Biological Factors Cytology Environmental Monitoring Genetics Irritants Microbiology Occupational Health Particulate Matter Respiratory System Risk Assessment Risk Factors Safety Workplace

  More +
• Keywords:
  Aneuploidy Author Keywords: Spindle Biological-effects Biological-factors Cell-biology Cell-damage Cellular-reactions Exposure-assessment Exposure-levels Exposure-methods Genetic-disorders Humans Inhalation-studies Microscopic-analysis Microscopy Nanoparticles Nanotechnology Particle-aerodynamics Particulates Respiratory-irritants Risk-factors Statistical-analysis Work-environment Workplace-studies

  More +
• ISSN:
  0893-6692
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  50
• Issue:
  8
• NIOSHTIC Number:
  nn:20036000
• Citation:
  Environ Mol Mutagen 2009 Aug; 50(8):708-717
• Contact Point Address:
  Linda M. Sargent, Toxicology and Molecular Biology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, Mailstop L-3014, Morgantown, WV 26505
• Email:
  lqs1@cdc.gov
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Environmental and Molecular Mutagenesis
• End Date:
  20160630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2f27f9e037051292987e87feae0a15919853bd4040b6e8510ffc54b6caea5b4c6e2c8d2be1264f52a41e14673401171d1a29c99275c75768a26419d73682bd9f
• Download URL:
  https://stacks.cdc.gov/view/cdc/187630/cdc_187630_DS1.pdf
• File Type:
  [PDF - 397.21 KB ]