Quantitative Identification of Atrazine and Its Chlorinated Metabolites in Plasma

Details

• Personal Author:
  Andersen ME ; Brzezicki JM ; Cranmer BK ; Tessari JD
• Description:
  The objective of this study was to develop an analytical method to detect and quantitate the chlorotriazine herbicide atrazine (ATRA), and its chlorinated metabolites [desethylatrazine (DE-ATRA), desisopropylatrazine (DI-ATRA), and diaminochlorotriazine (DACT)] in plasma. Control plasma separated from whole rat blood was fortified with known concentrations of ATRA, DE-ATRA, DI-ATRA, and DACT. These compounds were extracted from the plasma using a liquid-liquid extraction technique, and the resulting extracts were derivatized with tetrabutyl ammonium hydroxide and methyl iodide to produce methylated derivatives of ATRA and its chlorinated metabolites. Derivatized samples and standards were analyzed using gas chromatography-mass spectrometry with selected ion monitoring. Recoveries of fortified plasma samples ranged from 84% to 97% and were validated to 100 ng/mL. This analytical method was subsequently verified in a small-scale animal study to determine time course concentrations of chlorotriazines in plasma following a single oral gavage dose of ATRA to female Sprague Dawley rats. [Description provided by NIOSH]
• Subjects:
  Animals Blood Chemistry Chromatography, Gas Genetics Hematologic Tests Hypersensitivity Mass Spectrometry Neoplasms Occupational Health Risk Assessment Risk Factors Safety

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• Keywords:
  Animal-studies Blood-analysis Blood-plasma Blood-samples Blood-sampling Blood-serum Blood-tests Cancer Cancer-rates Chemical-composition Chemical-hypersensitivity Chemical-indicators Chemical-properties Chemical-reactions Chemical-structure Chemical-synthesis Exposure-levels Exposure-limits Exposure-methods Gas-chromatography Gas-detectors Gas-liquid-chromatography Gene-mutation Genes Genetic-disorders Genetic-factors Genotoxic-effects Genotoxicity Mass-spectrometry Risk-analysis Risk-factors

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• ISSN:
  0146-4760
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; OSHA Region 8
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  27
• Issue:
  8
• NIOSHTIC Number:
  nn:20035768
• Citation:
  J Anal Toxicol 2003 Nov-Dec; 27(8):569-573
• Contact Point Address:
  J. M. Brzezicki, Department of Environmental and Radiological Health Sciences, Colorado State University, Ft Collins, Colorado 80523
• Email:
  jbrz@colostate.edu
• CAS Registry Number:
  Atrazine (CAS RN 1912-24-9)
• Federal Fiscal Year:
  2004
• Performing Organization:
  Colorado State University - Fort Collins
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Journal of Analytical Toxicology
• End Date:
  20030929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0db4af06bd9a58b1f98117b22e42a9c647fe72f6bdf6618e7034eb889060e1f18064cc3ccec98bc6a3a51a7a0a597d9bd3ebb3fc96d10f0a09eecedf49d0b0b8
• Download URL:
  https://stacks.cdc.gov/view/cdc/187505/cdc_187505_DS1.pdf
• File Type:
  [PDF - 792.23 KB ]